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PMID: 3014865 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regional localization of DNA sequences on chromosome 21 using somatic cell hybrids.

American journal of human genetics ·Vol. 38 ·No. 6 ·1986-06-00 ·Pages 793-804

Van Keuren ML, Watkins PC, Drabkin HA, Jabs EW, Gusella JF, Patterson D

Abstract

We have used a panel of Chinese hamster X human somatic cell hybrids, each containing various portions of chromosome 21 as the only detectable human chromosome component, for regional mapping of cloned, chromosome 21-derived DNA sequences. Thirty unique and very low-repeat sequences were mapped to the short arm and three sections of the long arm. Three unique sequences map to the proximal part of the terminal band 21q22.3, and five to the distal part of this band. Some of these may represent parts of gene sequences that may be relevant to the pathogenesis of Down syndrome, as 21q22 is the area required to be present in triplicate for the full clinical picture.

MeSH Terms
Animals Base Sequence Chromosome Mapping Chromosomes, Human, 21-22 and Y Cloning, Molecular Cricetinae Cricetulus DNA/genetics DNA Restriction Enzymes Down Syndrome/genetics Genetic Markers Humans Hybrid Cells Mice
Chemicals
Genetic Markers DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Van Keuren M L
Watkins P C
Drabkin H A
Jabs E W
Gusella J F
Patterson D
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1986-06-00
Pages
793-804
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1684850
Subset
IM
Grants
NICHD NIH HHS · F32 HD06470 · United States
NICHD NIH HHS · HD17441 · United States
NICHD NIH HHS · HD17449 · United States
External Links
PubMed source PMC Full Text View journal details
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