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PMID: 3862098 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete structure of the alpha B-crystallin gene: conservation of the exon-intron distribution in the two nonlinked alpha-crystallin genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 17 ·1985-09-00 ·Pages 5819-23

Quax-Jeuken Y, Quax W, van Rens G, Khan PM, Bloemendal H

Abstract

We isolated bovine complementary DNA clones for the alpha A- and alpha B-crystallin subunits. The alpha B cDNA clone was used to isolate an alpha B-crystallin gene. This gene, derived from hamster, occurs as a single copy in the genome and is 3.2 kilobases long. The coding sequences are spread on three exons with a total length of 709 nucleotides. The exon-intron distribution of the hamster alpha B-crystallin gene is similar to that of the alpha A-crystallin gene except for the 69 nucleotides that specify the 23 "insert" residues of the alpha AIns chain by means of differential splicing. The 3' noncoding region of the alpha B mRNA (140 bases), which is short compared with the alpha A mRNA (520 bases), shows a remarkable homology between calf and hamster. Both alpha-crystallin cDNA clones have been used to assign the chromosomal location of the corresponding human genes with the aid of somatic cell hybrids. It is shown that the single-copy alpha A- and alpha B-crystallin genes are located on different chromosomes.

MeSH Terms
Animals Base Sequence Cattle Chromosomes, Human, 16-18 Chromosomes, Human, 21-22 and Y Cloning, Molecular Cricetinae Crystallins/genetics DNA/genetics Gene Expression Regulation Genes Humans Poly A/genetics Promoter Regions, Genetic
Chemicals
Crystallins Poly A DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Quax-Jeuken Y
Quax W
van Rens G
Khan P M
Bloemendal H
References (38)
38 references, click to expand
  1. Electrophoretic characterization and genetics of human biliverdin reductase (BLVR; EC 1.3.1.24); assignment of BLVR to the p14 leads to cen region of human chromosome 7 in mouse-human somatic cell hybrids.
    Biochem Genet. 1983 Feb;21(1-2):123-33 PMID: 6838484
  2. Internally elongated rodent alpha-crystallin A chain: resulting from incomplete RNA splicing?
    Biochem Biophys Res Commun. 1980 Sep 30;96(2):648-55 PMID: 7426005
  3. The amino-acids sequence of the alphaB2 chain of bovine alpha-crystallin.
    Eur J Biochem. 1974 Nov 1;49(1):157-68 PMID: 4477528
  4. Construction of coliphage lambda Charon vectors with BamHI cloning sites.
    Gene. 1980 Dec;12(3-4):301-9 PMID: 6265323
  5. The chicken delta-crystallin gene family. Two genes of similar structure in close chromosomal approximation.
    J Biol Chem. 1984 Aug 10;259(15):9821-5 PMID: 6746668
  6. The evolution of genes: the chicken preproinsulin gene.
    Cell. 1980 Jun;20(2):555-66 PMID: 7388949
  7. Basic defect in the expression of adenosine deaminase in ADA- SCID disease investigated through the cells of an obligate heterozygote.
    Hum Genet. 1981;56(3):379-86 PMID: 7239521
  8. Lens proteins.
    CRC Crit Rev Biochem. 1982;12(1):1-38 PMID: 7037295
  9. Loss of human genetic markers in man--Chinese hamster somatic cell hybrids.
    Nat New Biol. 1971 Nov 3;234(44):20-4 PMID: 5286856
  10. Cloning and analysis of cDNA sequences coding for two 16 kilodalton heat shock proteins (hsps) in Caenorhabditis elegans: homology with the small hsps of Drosophila.
    Nucleic Acids Res. 1983 May 25;11(10):3187-205 PMID: 6190129
  11. The structure of the vimentin gene.
    Cell. 1983 Nov;35(1):215-23 PMID: 6194898
  12. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  13. Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions.
    Mol Cell Biol. 1983 Oct;3(10):1738-45 PMID: 6646120
  14. Assignment of adenosine deaminase complexing protein (ADCP) gene(s) to human chromosome 2 in rodent-human somatic cell hybrids.
    Hum Genet. 1981;59(4):317-23 PMID: 6120891
  15. Molecular cloning of mRNA sequences encoding rat lens crystallins.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5320-4 PMID: 6946472
  16. Lens-specific expression of the chloramphenicol acetyltransferase gene promoted by 5' flanking sequences of the murine alpha A-crystallin gene in explanted chicken lens epithelia.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2334-8 PMID: 3857584
  17. Human formaldehyde dehydrogenase (FDH) and its assignment to chromosome 4.
    Cytogenet Cell Genet. 1984;38(2):112-5 PMID: 6467984
  18. Bovine beta-crystallin complementary DNA clones. Alternating proline/alanine sequence of beta B1 subunit originates from a repetitive DNA sequence.
    J Mol Biol. 1984 Dec 15;180(3):457-72 PMID: 6527379
  19. Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal genes: 3' untranslated regions are isotype specific but are conserved in evolution.
    Mol Cell Biol. 1983 Oct;3(10):1783-91 PMID: 6646124
  20. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  21. The integration sites of endogenous and exogenous Moloney murine leukemia virus.
    Cell. 1979 Sep;18(1):109-16 PMID: 509516
  22. Rat alpha-crystallin A chain with an insertion of 22 residues.
    Eur J Biochem. 1978 Aug 15;89(1):259-66 PMID: 699911
  23. alpha A-crystallin messenger RNA of the mouse lens: more noncoding than coding sequences.
    Science. 1982 Feb 19;215(4535):985-7 PMID: 7156978
  24. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
    Nucleic Acids Res. 1982 May 11;10(9):2951-61 PMID: 7099970
  25. The absence of the long 3'-non-translated region in mRNA coding for eye lens alpha A2-crystallin of the frog (Rana temporaria).
    FEBS Lett. 1983 Oct 3;162(1):47-51 PMID: 6604666
  26. The vertebrate eye lens.
    Science. 1977 Jul 8;197(4299):127-38 PMID: 877544
  27. A strategy of DNA sequencing employing computer programs.
    Nucleic Acids Res. 1979 Jun 11;6(7):2601-10 PMID: 461197
  28. Alternative RNA splicing of the murine alpha A-crystallin gene: protein-coding information within an intron.
    Cell. 1983 Mar;32(3):707-12 PMID: 6187470
  29. Direct assignment of citrate synthase (CS) gene to human chromosome 12 in man-mouse somatic cell hybrids.
    Hum Genet. 1977 Dec 23;39(3):339-44 PMID: 598842
  30. Delta crystallins and their nucleic acids.
    Mol Cell Biochem. 1984;59(1-2):33-56 PMID: 6369110
  31. The primary structure of the B2 chain of human alpha-crystallin.
    FEBS Lett. 1977 Feb 15;74(1):82-4 PMID: 838078
  32. The DNA sequence analysis of soybean heat-shock genes and identification of possible regulatory promoter elements.
    EMBO J. 1984 Nov;3(11):2491-7 PMID: 16453563
  33. Speculations on RNA splicing.
    Cell. 1981 Mar;23(3):643-6 PMID: 7226224
  34. The human PGM-2 and its chromosomal localization in man-mouse hybrids.
    Hum Genet. 1977 Jul 26;37(3):271-8 PMID: 885546
  35. Four small Drosophila heat shock proteins are related to each other and to mammalian alpha-crystallin.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2360-4 PMID: 6285380
  36. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  37. Why genes in pieces?
    Nature. 1978 Feb 9;271(5645):501 PMID: 622185
  38. Heterotopic chondrogenesis and osteogenesis induced by transformed cells: use of nude mice as a model system.
    Somatic Cell Genet. 1975 Oct;1(4):391-5 PMID: 194348
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.17.5819
Published
1985-09-00
Pages
5819-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390644
Subset
IM
Databases
GENBANK
M12014, M12015, M12016
External Links
PubMed source DOI: 10.1073/pnas.82.17.5819 PMC Full Text View journal details
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