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PMID: 6589633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a cloned DNA sequence that is present at centromeres of all human autosomes and the X chromosome and shows polymorphic variation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 15 ·1984-08-00 ·Pages 4884-8

Jabs EW, Wolf SF, Migeon BR

Abstract

We have identified a human DNA recombinant (p308) with a 3.0-kilobase (kb) BamHI insert that hybridizes in situ exclusively to the centromeric region of all human autosomes and the X chromosome. This highly repetitive sequence is significantly enriched on several chromosomes, most prominently on chromosome 6. In all individuals, the majority of genomic repeats are organized as tandem 3.0-kb BamHI repeats, each containing one Taq I site; the others are organized into BamHI and Taq I repeats of variable size that have some chromosome specificity. Using mouse-human hybrids, we have defined the specific organization of this sequence on chromosomes 6, 3, and X. In some individuals, there are differences in the number and nature of the tandem repeats. These polymorphisms segregate in families as if chromosome specific. Although variable from one chromosome to another, 308 contains sequences homologous to DNA present in centric heterochromatin of essentially all human chromosomes and is evolutionarily conserved. Therefore, a significant component of pericentric DNA is similar for all human chromosomes.

MeSH Terms
Base Sequence Biological Evolution Centromere/ultrastructure Chromosomes/ultrastructure Cloning, Molecular Female Humans Nucleic Acid Hybridization Polymorphism, Genetic Repetitive Sequences, Nucleic Acid X Chromosome/ultrastructure
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jabs E W
Wolf S F
Migeon B R
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.15.4884
Published
1984-08-00
Pages
4884-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391596
Subset
IM
Grants
NICHD NIH HHS · HD 05465 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.15.4884 PMC Full Text View journal details
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