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PMID: 4047940 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of polymorphic DNA segments from human chromosome 21.

Nucleic acids research ·Vol. 13 ·No. 17 ·1985-09-11 ·Pages 6075-88

Watkins PC, Tanzi RE, Gibbons KT, Tricoli JV, Landes G, Eddy R, Shows TB, Gusella JF

Abstract

A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction fragment length polymorphisms to provide genetic markers on chromosome 21. Nine independently isolated clones used as probes identified a total of 11 new RFLPs. Four of the DNA probes recovered from the library have been mapped unequivocally to chromosome 21 using a panel of somatic cell hybrid lines. A fifth probe detected an RFLP on chromosome 21 as well as sequences on other chromosomes. This set of RFLPs may now form the basis for construction of a genetic linkage map of human chromosome 21.

MeSH Terms
Animals Base Sequence Cell Line Chromosomes, Human, 21-22 and Y Cloning, Molecular DNA/isolation & purification DNA, Recombinant/isolation & purification Female Humans Hybrid Cells/cytology Male Mice Nucleic Acid Hybridization Pedigree Polymorphism, Genetic Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Watkins P C
Tanzi R E
Gibbons K T
Tricoli J V
Landes G
Eddy R
Shows T B
Gusella J F
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.17.6075
Published
1985-09-11
Pages
6075-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321939
Subset
IM
Grants
NIGMS NIH HHS · GM20454 · United States
NINDS NIH HHS · NS16367 · United States
NINDS NIH HHS · NS20012 · United States
External Links
PubMed source DOI: 10.1093/nar/13.17.6075 PMC Full Text View journal details
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