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PMID: 2999696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data.

Nucleic acids research ·Vol. 13 ·No. 21 ·1985-11-11 ·Pages 7551-68

Tomaschewski J, Gram H, Crabb JW, Rüger W

Abstract

Bacteriophage T4 alpha- and beta-glucosyltransferases link glucosyl units to the 5-HMdC residues of its DNA. The monoglucosyl group in alpha-linkage predominates over the one in beta linkage. Having recently reported on the nucleotide sequence of gene alpha gt (1) we now determined the nucleotide sequence of gene beta gt. The genes were each cloned on a high expression vector under the control of the lambda pL promoter. After thermo-induction the proteins were isolated and purified to homogeneity. To verify that the translational starting sites and the proposed reading frames are effective in vivo the sequence of the first 31 amino acid residues from gp alpha gt and the first 30 amino acid residues from gp beta gt were determined by Edman degradation. The primary structures of the two proteins seem to have only limited structural similarities. The results are discussed comparing secondary structure predictions and homologies with other proteins from the protein sequence database of the Protein Identification Resource.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Codon DNA Restriction Enzymes Escherichia coli/enzymology,genetics Genes Genes, Viral Genetic Vectors Glucosyltransferases/genetics Plasmids T-Phages/enzymology,genetics
Chemicals
Codon Glucosyltransferases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tomaschewski J
Gram H
Crabb J W
Rüger W
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62 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.21.7551
Published
1985-11-11
Pages
7551-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322070
Subset
IM
Databases
GENBANK
K03111
External Links
PubMed source DOI: 10.1093/nar/13.21.7551 PMC Full Text View journal details
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