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PMID: 4018026 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.

The EMBO journal ·Vol. 4 ·No. 1 ·1985-01-00 ·Pages 257-64

Gram H, Rüger W

Abstract

The nucleotide sequence of T4 genes 55, alpha gt, 47 and 46 was determined by a combination of 'classical' procedures and a shotgun approach. Small DNA fragments generated by frequent cleavage with restriction enzymes or by sonication of restriction fragments were cloned in phage M13 vectors and sequenced by the dideoxy method. The positions of the genes were determined by marker rescue between the corresponding T4 amber mutants and the cloned T4 DNA fragments used in the sequencing experiments. The sequence gives an insight into the organization of this 7.1-kb early region of the T4 genome and shows that genetically 'silent' portions within this region are not void of genetic information.

MeSH Terms
Base Sequence Chromosome Mapping Cloning, Molecular DNA, Viral/genetics Genes, Viral Operon Protein Biosynthesis T-Phages/genetics Transcription, Genetic
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gram H
Rüger W
References (62)
62 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-01-00
Pages
257-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554178
Subset
IM
Databases
GENBANK
J02510, M10160, X00769
External Links
PubMed source PMC Full Text View journal details
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