Home LiteratureArticle Details
PMID: 6370680 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence and cloning of bacteriophage T4 gene 63 encoding RNA ligase and tail fibre attachment activities.

The EMBO journal ·Vol. 3 ·No. 2 ·1984-02-00 ·Pages 397-402

Rand KN, Gait MJ

Abstract

The sequence of gene 63 of bacteriophage T4 was determined by a shotgun approach. Small DNA fragments, derived by sonication of a restriction fragment that encompasses the region of gene 63, were cloned in M13 vectors and sequenced by the 'dideoxy' method. The position of the gene was established by comparison with the sequence of a gene 63 amber mutant. Knowledge of the DNA sequence of gene 63 and surrounding regions has allowed the construction of a clone of gene 63 in which RNA ligase production is under the control of the lac promoter of bacteriophage M13mp8. Infected E. coli cells can be induced to produce a protein indistinguishable from commercially available RNA ligase.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Recombinant DNA, Viral/genetics Escherichia coli/genetics Genes, Viral Morphogenesis Polynucleotide Ligases/genetics RNA Ligase (ATP)/biosynthesis,genetics T-Phages/genetics,growth & development Viral Proteins/genetics
Chemicals
DNA, Recombinant DNA, Viral Viral Proteins Polynucleotide Ligases RNA Ligase (ATP)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rand K N
Gait M J
References (52)
52 references, click to expand
  1. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.
    J Mol Biol. 1966 Mar;16(1):118-33 PMID: 5331240
  2. Inhibition of the synthesis of beta-galactosidase in Escherichia coli by 2-nitrophenyl-beta-D-fucoside.
    J Mol Biol. 1966 Jul;18(2):339-43 PMID: 5338758
  3. Attachment of tail fibers in bacteriophage T4 assembly: some properties of the reaction in vitro and its genetic control.
    J Mol Biol. 1969 Feb 14;39(3):603-18 PMID: 5357214
  4. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
    Virology. 1970 Mar;40(3):734-44 PMID: 4908735
  5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  6. Purification and properties of bacteriophage T4-induced RNA ligase.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):3009-13 PMID: 4342972
  7. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  8. T4-induced RNA ligase joins single-stranded oligoribonucleotides.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):122-6 PMID: 1090929
  9. Catalysis of DNA joining by bacteriophage T4 RNA ligase.
    Biochem Biophys Res Commun. 1976 Jan 26;68(2):417-24 PMID: 766755
  10. Purification and properties of bacteriophage T4-induced RNA ligase.
    Arch Biochem Biophys. 1976 May;174(1):167-76 PMID: 938049
  11. A gene of bacteriophage T4 whose product prevents true late transcription on cytosine-containing T4 DNA.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3098-102 PMID: 1067605
  12. The genome of bacteriophage T4.
    Bacteriol Rev. 1976 Dec;40(4):847-68 PMID: 795415
  13. T4 Bacteriophage-coded RNA polymerase subunit blocks host transcription and unfolds the host chromosome.
    Nature. 1977 Jan 6;265(5589):28-32 PMID: 834238
  14. Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates.
    J Biol Chem. 1977 Mar 10;252(5):1732-8 PMID: 320212
  15. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  16. Bacteriophage T4 RNA ligase: preparation of a physically homogeneous, nuclease-free enzyme from hyperproducing infected cells.
    Nucleic Acids Res. 1977 Sep;4(9):3175-86 PMID: 333400
  17. Bacteriophage T4 RNA ligase is gene 63 product, the protein that promotes tail fiber attachment to the baseplate.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3355-9 PMID: 333436
  18. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  19. Isolation of a bacterial host selective for bacteriophage T4 containing cytosine in its DNA.
    J Virol. 1978 Sep;27(3):815-8 PMID: 359832
  20. Reactions at the termini of tRNA with T4 RNA ligase.
    Nucleic Acids Res. 1978 Oct;5(10):3665-77 PMID: 724498
  21. Purification of T4 RNA ligase by 2', 5'-ADP sepharose chromatography.
    FEBS Lett. 1979 Jan 1;97(1):73-6 PMID: 761619
  22. The purification of nuclease-free T4-RNA ligase.
    Biochim Biophys Acta. 1979 Mar 28;562(1):149-61 PMID: 219895
  23. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  24. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  25. Molecular cloning of the DNA ligase gene from bacteriophage T4. I. Characterisation of the recombinants.
    J Mol Biol. 1979 Aug 15;132(3):471-91 PMID: 160464
  26. Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.
    J Mol Biol. 1979 Aug 15;132(3):493-505 PMID: 392109
  27. RNA ligase reaction products in plasmolyzed Escherichia coli cells infected by T4 bacteriophage.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5430-4 PMID: 392502
  28. A simple and fast electrophoretic method for elution of nucleic acids from gels.
    Mol Biol Rep. 1979 Dec 31;5(4):237-9 PMID: 530273
  29. T4 ribonucleic acid ligase joins single-strand oligo(deoxyribonucleotides).
    Biochemistry. 1980 Feb 19;19(4):635-42 PMID: 6986903
  30. DNA sequence of a T4 transfer RNA gene cluster.
    J Mol Biol. 1980 May 25;139(3):377-91 PMID: 6255163
  31. Molecular cloning of the T4 genome; organization and expression of the frd--DNA ligase region.
    Mol Gen Genet. 1980;179(2):227-39 PMID: 6258011
  32. A restriction map of the bacteriophage T4 genome.
    Mol Gen Genet. 1980;179(2):421-435 PMID: 6258018
  33. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  34. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  35. Genetic and physiological studies of the role of the RNA ligase of bacteriophage T4.
    J Mol Biol. 1982 Jan 15;154(2):273-86 PMID: 7042981
  36. Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2997-3011 PMID: 7048259
  37. The making of strand-specific M13 probes.
    Gene. 1982 Mar;17(3):271-7 PMID: 7049837
  38. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
    Gene. 1982 Jun;18(3):199-209 PMID: 6751939
  39. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  40. Purification of T4 RNA ligase by dextran blue-Sepharose 4B affinity chromatography.
    Anal Biochem. 1982 Sep 1;125(1):1-5 PMID: 7149245
  41. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  42. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  43. Bacteriophage T4-induced anticodon-loop nuclease detected in a host strain restrictive to RNA ligase mutants.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7097-101 PMID: 6296815
  44. Sensitive detection of RNA using strand-specific M13 probes.
    Gene. 1982 Dec;20(2):139-44 PMID: 6187624
  45. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  46. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis.
    Anal Biochem. 1983 Feb 15;129(1):216-23 PMID: 6305233
  47. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  48. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1983 Jan 11;11(1):r135-67 PMID: 6306557
  49. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  50. Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):6105-9 PMID: 6310615
  51. Using T4 RNA ligase with DNA substrates.
    Methods Enzymol. 1983;100:38-52 PMID: 6353144
  52. Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.
    EMBO J. 1982;1(11):1399-404 PMID: 6327267
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-02-00
Pages
397-402
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557357
Subset
IM
Databases
GENBANK
X00365
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2