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PMID: 1067605 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A gene of bacteriophage T4 whose product prevents true late transcription on cytosine-containing T4 DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 9 ·1976-09-00 ·Pages 3098-102

Snyder L, Gold L, Kutter E

Abstract

T-even coliphages have 5-hydroxymethylcytosine in their DNA instead of cytosine. In some T4 mutants, the replicated DNA contains cytosine, but then no late gene products are made. We show that the inability to make late gene products with cytosine-containing T4 DNA is due to a T4 gene products. This gene product, while probably nonessential under normal conditions, interacts with an essential part of the transcription apparatus. Mutations in this gene allow viable T4 particles to be made whose DNA has been substituted almost 100% with cytosine.

MeSH Terms

Coliphages/metabolism Cytosine/metabolism DNA Restriction Enzymes/metabolism DNA, Viral/metabolism Genes Genes, Recessive Mutation RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Templates, Genetic Time Factors Transcription, Genetic

Chemicals

DNA, Viral RNA, Messenger RNA, Viral Cytosine DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Snyder L

Gold L

Kutter E

References (24)

24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.9.3098

Published

1976-09-00

Pages

3098-102

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430943

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.9.3098 PMC Full Text View journal details

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A gene of bacteriophage T4 whose product prevents true late transcription on cytosine-containing T4 DNA.

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