Template properties of glucose-deficient T-even bacteriophage DNA.

Journal of virology ·Vol. 12 ·No. 6 ·1973-12-00 ·Pages 1279-87

Cox GS, Conway TW

Abstract

The vegetative DNA isolated from T4-infected Escherichia coli W4597 (UDPG PPase(-)) was about two to six times more active in stimulating protein synthesis in cell-free extracts than that isolated from T4-infected E. coli B06. This suggested that nonglucosylated vegetative DNA may be a better template than the glucosylated form. This view was supported by experiments measuring RNA synthesis on mature T-even DNAs with a range of glucose contents. The extent of (14)C-GTP polymerization was inversely proportional to the glucose content of the DNA. Differences were also observed in both the kind and quantity of polypeptides produced in response to these DNAs.

MeSH Terms

Amino Acids/metabolism Autoradiography Carbon Radioisotopes Cell-Free System Coliphages/analysis,growth & development DNA, Viral/analysis Electrophoresis, Polyacrylamide Gel Escherichia coli Glucose/analysis Guanosine Triphosphate/metabolism Mutation RNA, Viral/biosynthesis Shigella dysenteriae Templates, Genetic Transcription, Genetic Viral Proteins/biosynthesis Virus Replication

Chemicals

Amino Acids Carbon Radioisotopes DNA, Viral RNA, Viral Viral Proteins Guanosine Triphosphate Glucose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cox G S

Conway T W

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23 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.12.6.1279-1287.1973

Published

1973-12-00

Pages

1279-87

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356769

Subset

External Links

PubMed source DOI: 10.1128/JVI.12.6.1279-1287.1973 PMC Full Text View journal details

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Template properties of glucose-deficient T-even bacteriophage DNA.

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