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PMID: 2985954 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Repeat arrays in cellular DNA related to the Epstein-Barr virus IR3 repeat.

Molecular and cellular biology ·Vol. 5 ·No. 3 ·1985-03-00 ·Pages 457-65

Heller M, Flemington E, Kieff E, Deininger P

Abstract

We isolated clones and determined the sequence of portions of mouse and human cellular DNA which cross-hybridize strongly with the IR3 repetitive region of Epstein-Barr virus. The sequences were found to be tandem arrays of a simple sequence based on the triplet GGA, very similar to the IR3 repeat. The cellular repeats have distinct differences from the viral repeat region, however, and their sequences do not appear capable of being translated into a purely glycine-plus-alanine protein domain like the portion of the Epstein-Barr nuclear antigen coded by IR3. Although the relationship between IR3 and the cellular repeats is left unclear, the cellular repeats have many interesting features. The tandem arrays are about 1 to several kilobases long, much shorter than satellite tandem repeats and larger than other interspersed, tandem repeats. Each of the repeats is a distinct variation, perhaps diverged from a common sequence, (GGA)n. This family is present in the genomes of all species tested and appears to be a ubiquitous feature of all higher eucaryotic genomes.

MeSH Terms
Animals Antigens, Viral/genetics Cloning, Molecular DNA, Recombinant DNA, Viral/genetics Epstein-Barr Virus Nuclear Antigens Genes, Viral Herpesvirus 4, Human/genetics Humans Mice Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Species Specificity
Chemicals
Antigens, Viral DNA, Recombinant DNA, Viral Epstein-Barr Virus Nuclear Antigens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Heller M
Flemington E
Kieff E
Deininger P
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27 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.3.457-465.1985
Published
1985-03-00
Pages
457-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366737
Subset
IM
Grants
NCI NIH HHS · CA17281 · United States
NCI NIH HHS · CA19264 · United States
NIGMS NIH HHS · GM29848 · United States
Databases
GENBANK
M10239, M10296, M10667, M10668, M17915, M17916, M17917, M17918, M17919, M17920
External Links
PubMed source DOI: 10.1128/mcb.5.3.457-465.1985 PMC Full Text View journal details
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