Home LiteratureArticle Details
PMID: 6285297 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Simple DNA sequences in homologous flanking regions near immunoglobulin VH genes: a role in gene interaction?

Nucleic acids research ·Vol. 10 ·No. 11 ·1982-06-11 ·Pages 3353-70

Cohen JB, Effron K, Rechavi G, Ben-Neriah Y, Zakut R, Givol D

Abstract

Five closely related immunoglobulin VH genes (subgroup II) were compared by sequencing of several kb of DNA. In three of the genes homology greater than 75% was found along an area of 4 kb that includes the coding region. The homology in flanking regions is only slightly lower than that in the coding sequences. Two other genes, which are located on the same EcoRI fragment, show high homology to the first three genes in the coding and immediately flanking regions. In more distant flanking regions no homology is found with the first three genes. This indicates that their evolutionary history differs from that of the other three genes. A region of simple DNA sequence composed of repetitive TCC and TCA elements was found at a distance of approximately 380 bp upstream from the initiator ATG of these VH genes. This region is the site where the two sets of genes abruptly start to diverge. The structure of the simple DNA sequence in the various VH genes suggests that it may be involved in gene interaction. We propose that both simple DNA sequences and homology in flanking regions serve a function in the correction of VH genes, which seem to be rather free to diverge and drift into pseudogenes. A correction mechanism may help this gene family to maintain its two major features, multiplicity and diversity.

MeSH Terms
Animals Base Composition Base Sequence Binding Sites, Antibody/genetics Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Recombinant/metabolism Embryo, Mammalian Genes Immunoglobulin Variable Region/genetics Mice Mice, Inbred BALB C Nucleic Acid Hybridization
Chemicals
DNA, Recombinant Immunoglobulin Variable Region DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cohen J B
Effron K
Rechavi G
Ben-Neriah Y
Zakut R
Givol D
References (38)
38 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  3. The organization, expression, and evolution of antibody genes and other multigene families.
    Annu Rev Genet. 1975;9:305-53 PMID: 813561
  4. The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.
    J Mol Biol. 1976 Aug 25;105(4):461-86 PMID: 787539
  5. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  6. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  7. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  8. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  9. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  10. Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing.
    Cell. 1978 Jul;14(3):655-71 PMID: 688387
  11. Antibody diversity.
    Science. 1978 Oct 6;202(4363):11-7 PMID: 99815
  12. The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions.
    Cell. 1978 Nov;15(3):1033-44 PMID: 728984
  13. Sequences of mouse immunoglobulin light chain genes before and after somatic changes.
    Cell. 1978 Dec;15(4):1133-44 PMID: 103630
  14. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  15. Histone genes and histone messengers.
    Annu Rev Biochem. 1979;48:837-70 PMID: 112914
  16. Effects of DNA sequence non-homology on formation of bacteriophage lambda recombinants.
    J Mol Biol. 1979 Jun 5;130(4):357-77 PMID: 384000
  17. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  18. The joining of V and J gene segments creates antibody diversity.
    Nature. 1980 Jan 31;283(5746):497-9 PMID: 6766210
  19. Contribution of immunoglobulin heavy-chain variable-region genes to antibody diversity.
    Nature. 1980 Mar 20;284(5753):238-43 PMID: 6767191
  20. Organization and complete sequence of identical embryonic and plasmacytoma kappa V-region genes.
    J Biol Chem. 1980 Apr 25;255(8):3691-4 PMID: 6767723
  21. Rapid duplication and loss of genes coding for the alpha chains of hemoglobin.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2158-62 PMID: 6929543
  22. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  23. The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions.
    Cell. 1980 May;20(1):119-30 PMID: 6446404
  24. The structure of a human alpha-globin pseudogene and its relationship to alpha-globin gene duplication.
    Cell. 1980 Sep;21(2):537-44 PMID: 7407925
  25. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  26. Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
    Nucleic Acids Res. 1980 May 24;8(10):2255-74 PMID: 6159575
  27. Cloning and sequence of the cDNA corresponding to the variable region of immunoglobulin heavy chain MPC11.
    Nucleic Acids Res. 1980 Aug 25;8(16):3591-601 PMID: 6253904
  28. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.
    Cell. 1980 Oct;21(3):627-38 PMID: 7438203
  29. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
  30. Structure of immunoglobulin gamma 2b heavy chain gene cloned from mouse embryo gene library.
    Nucleic Acids Res. 1980 Feb 11;8(3):453-66 PMID: 6255443
  31. Human immunoglobulin variable region genes--DNA sequences of two V kappa genes and a pseudogene.
    Nature. 1980 Dec 25;288(5792):730-3 PMID: 6779204
  32. Homology and concerted evolution at the alpha 1 and alpha 2 loci of human alpha-globin.
    Nature. 1981 Mar 5;290(5801):26-9 PMID: 7010180
  33. Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):509-13 PMID: 6787590
  34. Gene conversion: some implications for immunoglobulin genes.
    Cell. 1981 Jun;24(3):592-4 PMID: 7018693
  35. Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.
    Cell. 1981 Jun;24(3):625-37 PMID: 6788376
  36. Diversity of germ-line immunoglobulin VH genes.
    Nature. 1981 Jul 30;292(5822):426-30 PMID: 6789211
  37. A single VH gene segment encodes the immune response to phosphorylcholine: somatic mutation is correlated with the class of the antibody.
    Cell. 1981 Jul;25(1):59-66 PMID: 6791833
  38. Antibody diversity: somatic hypermutation of rearranged VH genes.
    Cell. 1981 Dec;27(3 Pt 2):573-81 PMID: 6101208
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.11.3353
Published
1982-06-11
Pages
3353-70
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320716
Subset
IM
Databases
GENBANK
J00487, J00489, J00490, J00503, J00504, J00505, J00506, J00507, J00508, J00509, J00511, J00512, J00513, J00514
External Links
PubMed source DOI: 10.1093/nar/10.11.3353 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2