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PMID: 6292493 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Simple repeat sequence in Epstein-Barr virus DNA is transcribed in latent and productive infections.

Journal of virology ·Vol. 44 ·No. 1 ·1982-10-00 ·Pages 311-20

Heller M, van Santen V, Kieff E

Abstract

The BamHI K region of Epstein-Barr virus DNA is transcribed in latently infected cells from Burkitt tumors and in growth-transformed B-lymphocytes latently infected with Epstein-Barr virus. We determined the nucleotide sequence of a 1,153-base pair HinfI fragment in BamHI fragment K from the B95-8 Epstein-Barr virus isolate. The fragment contains a remarkable 708-base pair simple sequence repeat array, designated IR3, which is composed of only three nucleotide triplet elements: GGG, GCA, and GGA. The triplets are organized into three repeat units: GCAGGA, GCAGGAGGA, and GGGGCAGGA. Immediately 3' of IR3 are tandem nearly perfect direct repeats of two different 24-base pair sequences. IR3 is conserved at a colinear position in the DNAs of other Epstein-Barr virus isolates, and a homologous sequence maps at the same location in the genome of a genetically related baboon herpesvirus, herpesvirus papio. IR3 is transcribed from left to right in latently infected, growth-transformed IB4 cells. It encodes part of a 2.0-kilobase exon of the 3.7-kilobase cytoplasmic polyadenylated RNA previously detected in IB4 cells (van Santen et al., Proc. Natl. Acad. Sci. U.S.A. 78:1930-1934, 1981). IR3 also encodes parts of 2.4- and 1.0-kilobase RNAs in productively infected B95-8 cells.

MeSH Terms
Cell Line Cell Transformation, Viral DNA Restriction Enzymes DNA, Viral/genetics Deoxyribonuclease BamHI Herpesviridae/genetics Herpesvirus 4, Human/genetics,growth & development Humans RNA, Viral/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Viral RNA, Viral DNA Restriction Enzymes Deoxyribonuclease BamHI
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heller M
van Santen V
Kieff E
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46 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.44.1.311-320.1982
Published
1982-10-00
Pages
311-20
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256266
Subset
IM
Grants
NCI NIH HHS · CA 09268 · United States
NCI NIH HHS · CA 17281 · United States
NCI NIH HHS · CA 19264 · United States
Databases
GENBANK
J02079
External Links
PubMed source DOI: 10.1128/JVI.44.1.311-320.1982 PMC Full Text View journal details
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