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PMID: 6208381 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and characterization of a cellular protein that cross-reacts with the Epstein-Barr virus nuclear antigen.

Journal of virology ·Vol. 52 ·No. 3 ·1984-12-00 ·Pages 833-8

Luka J, Kreofsky T, Pearson GR, Hennessy K, Kieff E

Abstract

A 62,000-dalton (62K) cell protein reacts with antisera to the 72K polypeptide of the Epstein-Barr virus nuclear antigen (EBNA) in immunoblots. This protein was initially detected in EBNA-negative as well as EBNA-positive cell lines with anti-EBNA-positive human sera. A monoclonal antibody raised against the 72K EBNA and an antiserum from a rabbit immunized with the glycine-alanine domain of EBNA also reacted with the cellular protein. The cellular protein was partially purified from Epstein-Barr virus genome-positive and -negative cell lines. Absorption experiments identified a shared antigenic determinant between the 72K EBNA and 62K cellular protein. A comparison of the 62K protein and EBNA by protease digestion did not reveal similar peptides.

MeSH Terms
Antibodies, Monoclonal Antigens, Viral/immunology Cell Line Cross Reactions Epitopes Epstein-Barr Virus Nuclear Antigens Herpesvirus 4, Human/immunology Humans Molecular Weight Peptide Fragments/analysis Proteins/immunology
Chemicals
Antibodies, Monoclonal Antigens, Viral Epitopes Epstein-Barr Virus Nuclear Antigens Peptide Fragments Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Luka J
Kreofsky T
Pearson G R
Hennessy K
Kieff E
References (18)
18 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.
    Int J Cancer. 1973 May;11(3):499-520 PMID: 4133943
  3. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  4. Studies on Epstein-Barr virus-related antigens. III. Purification of the virus-determined nuclear antigen (EBNA) from non-producer Raji cells.
    Int J Cancer. 1978 Dec;22(6):747-52 PMID: 82547
  5. Identification of an Epstein-Barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis.
    J Virol. 1981 Jun;38(3):996-1004 PMID: 7017165
  6. Identification of Epstein-Barr virus strain differences with monoclonal antibody to a membrane glycoprotein.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):616-20 PMID: 6176998
  7. Evidence for two classes of chromatin-associated Epstein-Barr virus-determined nuclear antigen.
    J Virol. 1982 Aug;43(2):555-65 PMID: 6180183
  8. Theory and methods for immunization in culture and monoclonal antibody production.
    J Immunol Methods. 1982 Sep 30;53(3):261-91 PMID: 6754815
  9. Simple repeat sequence in Epstein-Barr virus DNA is transcribed in latent and productive infections.
    J Virol. 1982 Oct;44(1):311-20 PMID: 6292493
  10. Partial purification of the Epstein-Barr virus nuclear antigen(s).
    J Biol Chem. 1983 Mar 25;258(6):3974-82 PMID: 6300065
  11. Simple repeat array in Epstein-Barr virus DNA encodes part of the Epstein-Barr nuclear antigen.
    Science. 1983 Jun 24;220(4604):1396-8 PMID: 6304878
  12. Repeat array in Epstein-Barr virus DNA is related to cell DNA sequences interspersed on human chromosomes.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5916-20 PMID: 6310555
  13. One of two Epstein-Barr virus nuclear antigens contains a glycine-alanine copolymer domain.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5665-9 PMID: 6310587
  14. Functional mapping of the Epstein-Barr virus genome: identification of sites coding for the restricted early antigen, the diffuse early antigen, and the nuclear antigen.
    Virology. 1983 Aug;129(1):188-98 PMID: 6310877
  15. India ink staining of proteins on nitrocellulose paper.
    Anal Biochem. 1983 Aug;133(1):157-62 PMID: 6195938
  16. The Epstein-Barr virus-determined nuclear antigen: a previously identified 48K component and higher-molecular-weight forms of the antigen are structurally related.
    Intervirology. 1983;20(4):213-22 PMID: 6317606
  17. Identification of Epstein-Barr nuclear antigen polypeptide in mouse and monkey cells after gene transfer with a cloned 2.9-kilobase-pair subfragment of the genome.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):43-7 PMID: 6320171
  18. A sensitive enzyme-linked immunosorbent assay (ELISA) against the major EBV-associated antigens. I. Correlation between ELISA and immunofluorescence titers using purified antigens.
    J Immunol Methods. 1984 Feb 24;67(1):145-56 PMID: 6321599
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.52.3.833-838.1984
Published
1984-12-00
Pages
833-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254603
Subset
IM
Grants
NCI NIH HHS · CA25340 · United States
External Links
PubMed source DOI: 10.1128/JVI.52.3.833-838.1984 PMC Full Text View journal details
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