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PMID: 2985827 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Human rhinovirus 14 infection of HeLa cells results in the proteolytic cleavage of the p220 cap-binding complex subunit and inactivates globin mRNA translation in vitro.

Journal of virology ·Vol. 54 ·No. 2 ·1985-05-00 ·Pages 634-8

Etchison D, Fout S

Abstract

One of the characteristics of picornavirus infection of cells in tissue culture is a specific inhibition of utilization of host cell mRNA for protein synthesis. In this study we show that human rhinovirus 14 is similar to poliovirus in that the inhibition of host cell translation that occurs during infection correlates with the proteolytic cleavage of an Mr 220,000 subunit of the cap-binding protein complex.

MeSH Terms
Carrier Proteins/metabolism Globins/biosynthesis HeLa Cells/microbiology Humans Molecular Weight Peptide Initiation Factors/isolation & purification Protein Biosynthesis RNA Cap-Binding Proteins Rhinovirus/genetics,metabolism
Chemicals
Carrier Proteins Peptide Initiation Factors RNA Cap-Binding Proteins Globins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Etchison D
Fout S
References (19)
19 references, click to expand
  1. On the structure of rhinovirus 1A.
    Virology. 1971 May;44(2):259-70 PMID: 4327717
  2. Picornaviral gene order: comparison of a rhinovirus with a cardiovirus.
    J Virol. 1973 Feb;11(2):341-4 PMID: 4347432
  3. Encephalomyocarditis virus infection of mouse plasmacytoma cells. I. Inhibition of cellular protein synthesis.
    J Virol. 1974 Sep;14(3):598-610 PMID: 4368674
  4. Poliovirus-induced inhibition of polypeptide initiation in vitro on native polyribosomes.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1834-8 PMID: 180525
  5. Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3040-4 PMID: 184455
  6. Comparison of initiation rates of encephalomyocarditis virus and host protein synthesis in infected cells.
    J Virol. 1978 Sep;27(3):640-7 PMID: 212586
  7. A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5'-terminal cap in mRNA.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4843-7 PMID: 217002
  8. Protein synthesis initiation factors from rabbit reticulocytes: purification, characterization, and radiochemical labeling.
    Methods Enzymol. 1979;60:15-35 PMID: 459896
  9. Alterations in initiation factor activity from poliovirus-infected HeLa cells.
    J Biol Chem. 1979 Nov 10;254(21):10973-8 PMID: 227856
  10. Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4345-9 PMID: 291969
  11. ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA.
    Nucleic Acids Res. 1981 Apr 10;9(7):1643-56 PMID: 7232223
  12. Inactivation of cap-binding proteins accompanies the shut-off of host protein synthesis by poliovirus.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3447-51 PMID: 6954488
  13. Inhibition of host translation in encephalomyocarditis virus-infected L cells: a novel mechanism.
    J Virol. 1982 Jul;43(1):250-61 PMID: 6287000
  14. Immunochemical characterization of mammalian protein synthesis initiation factors.
    Biochemistry. 1982 Aug 31;21(18):4206-12 PMID: 6751385
  15. Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.
    J Biol Chem. 1982 Dec 25;257(24):14806-10 PMID: 6294080
  16. New initiation factor activity required for globin mRNA translation.
    J Biol Chem. 1983 May 10;258(9):5804-10 PMID: 6853548
  17. Involvement of eukaryotic initiation factor 4A in the cap recognition process.
    J Biol Chem. 1983 Sep 25;258(18):11398-403 PMID: 6604056
  18. Systematic nomenclature of picornavirus proteins.
    J Virol. 1984 Jun;50(3):957-9 PMID: 6726891
  19. Demonstration in vitro that eucaryotic initiation factor 3 is active but that a cap-binding protein complex is inactive in poliovirus-infected HeLa cells.
    J Virol. 1984 Sep;51(3):832-7 PMID: 6088805
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.54.2.634-638.1985
Published
1985-05-00
Pages
634-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254840
Subset
IM
Grants
NIAID NIH HHS · AI-19921 · United States
NIGMS NIH HHS · GM-22135 · United States
External Links
PubMed source DOI: 10.1128/JVI.54.2.634-638.1985 PMC Full Text View journal details
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