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PMID: 184455 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 9 ·1976-09-00 ·Pages 3040-4

Golini F, Thach SS, Birge CH, Safer B, Merrick WC, Thach RE

Abstract

Encephalomyocarditis viral RNA has previously been shown to outcompete host cellular mRNA has for translation in vitro in crude and fractionated protein synthesizing systems. In the present communication it is shown that the competition is regulated by an initiation factor or complex of factors, and not the 40S initiation complex per se. The factor primarily involved is the murine equivalent of a component present in a partially purified preparation of rabbit initiation factor M3. Both the murine and rabbit factors are clearly messenger discriminatory.

MeSH Terms

Animals Cell-Free System Encephalomyocarditis virus/metabolism Globins/biosynthesis Mice Multiple Myeloma Peptide Chain Initiation, Translational Peptide Initiation Factors RNA, Messenger/metabolism RNA, Neoplasm/metabolism RNA, Viral/metabolism Ribosomes/metabolism

Chemicals

Peptide Initiation Factors RNA, Messenger RNA, Neoplasm RNA, Viral Globins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Golini F

Thach S S

Birge C H

Safer B

Merrick W C

Thach R E

References (10)

10 references, click to expand

Nonribosomal proteins associated with eukaryotic native small ribosomal subunits.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3392-6 PMID: 1059126
A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.
Proc Natl Acad Sci U S A. 1976 May;73(5):1559-63 PMID: 1064023
5'-terminal structure of poliovirus polyribosomal RNA is pUp.
Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30 PMID: 174102
The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
Proc Natl Acad Sci U S A. 1976 Feb;73(2):375-80 PMID: 174106
Specificity in initiation of protein synthesis in a fractionated mammalian cell-free system.
Nat New Biol. 1973 Apr 4;242(118):136-40 PMID: 4349564
Discrimination between messenger ribonucleic acids by a mammalian translation initiation factor.
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2139-44 PMID: 4352974
Encephalomyocarditis virus infection of mouse plasmacytoma cells. I. Inhibition of cellular protein synthesis.
J Virol. 1974 Sep;14(3):598-610 PMID: 4368674
Model for the regulation of mRNA translation applied to haemoglobin synthesis.
Nature. 1974 Oct 4;251(5474):385-8 PMID: 4421673
Initiation of eukaryotic protein synthesis: (Met-tRNA f -40S ribosome) initiation complex catalysed by purified initiation factors in the absence of mRNA.
Nat New Biol. 1973 Mar 14;242(115):35-8 PMID: 4512006
Evidence for role of m7G5'-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3.
Nature. 1976 May 27;261(5558):291-4 PMID: 944862

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.9.3040

Published

1976-09-00

Pages

3040-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430920

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.9.3040 PMC Full Text View journal details

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Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor.

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