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PMID: 4352974 Published · ppublish English Journal Article

Discrimination between messenger ribonucleic acids by a mammalian translation initiation factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 7 ·1973-07-00 ·Pages 2139-44

Nudel U, Lebleu B, Revel M

Abstract

A factor from rabbit-reticulocyte ribosome, which stimulates by 5-fold initiation of hemoglobin mRNA translation in Krebs ascites cell extracts, was purified to homogeneity. The factor, a protein of about 65,000 molecular weight in sodium dodecyl sulfate, discriminates between various mRNAs: it stimulates translation of alpha globin mRNA and of tobacco mosaic virus RNA, but has only a small effect on beta globin mRNA and no effect at all on Mengo virus RNA translation.

MeSH Terms
Animals Carcinoma, Krebs 2 Cell Line Cell-Free System Chromatography, DEAE-Cellulose Globins/biosynthesis Hemoglobins Kinetics Mengovirus Methionine/metabolism Mice Peptide Initiation Factors/isolation & purification Polyribosomes/analysis Protein Biosynthesis/drug effects RNA, Messenger/isolation & purification,metabolism RNA, Viral/metabolism Rabbits Reticulocytes/analysis Sulfur Isotopes Tricarboxylic Acids/pharmacology
Chemicals
Hemoglobins Peptide Initiation Factors RNA, Messenger RNA, Viral Sulfur Isotopes Tricarboxylic Acids Globins Methionine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nudel U
Lebleu B
Revel M
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.7.2139
Published
1973-07-00
Pages
2139-44
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433683
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.7.2139 PMC Full Text View journal details
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