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PMID: 212586 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparison of initiation rates of encephalomyocarditis virus and host protein synthesis in infected cells.

Journal of virology ·Vol. 27 ·No. 3 ·1978-09-00 ·Pages 640-7

Jen G, Birge CH, Thach RE

Abstract

The relative initiation rates for encephalomyocarditis virus mRNA and host mRNA's in infected cells were measured using two independent techniques. In both cases the results showed that viral mRNA initiates at a much higher rate than host mRNA'S. This difference was observed midway in the infectious cycle, well before virus-induced cytopathic effects (leakage of low-molecular-weight metabolites, failure to exclude trypan blue) were apparent. These results confirm that encephalomyocarditis viral mRNA is a more efficient initiator than host mRNA's in vivo, as has previously been demonstrated in in vitro experiments.

MeSH Terms
Animals Anisomycin/pharmacology Carcinoma, Krebs 2 Culture Techniques Cycloheximide/pharmacology Emetine/pharmacology Encephalomyocarditis virus/metabolism Molecular Weight Neoplasm Proteins/biosynthesis Peptide Biosynthesis Peptide Chain Elongation, Translational/drug effects Peptide Chain Initiation, Translational RNA, Messenger/metabolism RNA, Neoplasm/metabolism RNA, Viral/metabolism Viral Proteins/biosynthesis
Chemicals
Neoplasm Proteins RNA, Messenger RNA, Neoplasm RNA, Viral Viral Proteins Anisomycin Cycloheximide Emetine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jen G
Birge C H
Thach R E
References (23)
23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.27.3.640-647.1978
Published
1978-09-00
Pages
640-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC525852
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.27.3.640-647.1978 PMC Full Text View journal details
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