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PMID: 2894474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of adenovirus type 2 L1 RNA 3'-end formation in vivo and in vitro.

Journal of virology ·Vol. 62 ·No. 4 ·1988-04-00 ·Pages 1464-8

Hales KH, Birk JM, Imperiale MJ

Abstract

Downstream sequence requirements for efficient cleavage and polyadenylation at the adenovirus type 2 L1 poly(A) site were determined in vivo in 293 cells and in vitro by using RNA precursors in HeLa cell nuclear extracts. The two cleavage sites used were found to differ in sensitivity to 3'-end deletion in vivo and in vitro.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Cloning, Molecular Endonucleases HeLa Cells Humans Molecular Sequence Data Mutation Plasmids Poly A/genetics,metabolism RNA Precursors/genetics,metabolism RNA, Messenger/genetics,metabolism RNA, Viral/genetics,metabolism Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
RNA Precursors RNA, Messenger RNA, Viral Poly A Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hales K H
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
Birk J M
Imperiale M J
References (35)
35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.4.1464-1468.1988
Published
1988-04-00
Pages
1464-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253164
Subset
IM
Grants
NIGMS NIH HHS · GM34902 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.4.1464-1468.1988 PMC Full Text View journal details
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