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PMID: 2888109 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression and secretion of type beta transforming growth factor by activated human macrophages.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 17 ·1987-09-00 ·Pages 6020-4

Assoian RK, Fleurdelys BE, Stevenson HC, Miller PJ, Madtes DK, Raines EW, Ross R, Sporn MB

Abstract

Alveolar macrophages activated with concanavalin A and peripheral blood monocytes activated with lipopolysaccharide secrete type beta transforming growth factor (TGF-beta). There is minimal TGF-beta secretion in unactivated monocytes, even though TGF-beta mRNA is expressed in these cells at a level similar to that in activated, lipopolysaccharide-treated cultures. U937 lymphoma cells, which have monocytic characteristics, also express mRNA for TGF-beta. Freshly isolated monocytes, both control and lipopolysaccharide-treated, secrete an acid-labile binding protein that inhibits TGF-beta action. We conclude the following: (i) that expression of TGF-beta mRNA is unrelated to monocyte activation, (ii) that secretion of TGF-beta is induced by monocyte activation, and (iii) that cosecretion of TGF-beta and its monocyte/macrophage-derived binding protein may modulate growth factor action. In contrast, monocytic expression of other growth factor genes, such as the B chain of platelet-derived growth factor, is not constitutive and requires activation.

MeSH Terms
Cell Line Humans In Vitro Techniques Macrophage Activation Macrophages/metabolism Monocytes/metabolism Peptides/metabolism RNA, Messenger/metabolism Receptors, Cell Surface/metabolism Receptors, Transforming Growth Factor beta Transforming Growth Factors
Chemicals
Peptides RNA, Messenger Receptors, Cell Surface Receptors, Transforming Growth Factor beta Transforming Growth Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Assoian R K
Fleurdelys B E
Stevenson H C
Miller P J
Madtes D K
Raines E W
Ross R
Sporn M B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.17.6020
Published
1987-09-00
Pages
6020-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298999
Subset
IM
Grants
NHLBI NIH HHS · HL-18645 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.17.6020 PMC Full Text View journal details
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