Home LiteratureArticle Details
PMID: 2843742 Published · ppublish English Journal Article

Ataxia telangiectasia resists gene cloning: an account of parameters determining gene transfer into human recipient cells.

Molecular & general genetics : MGG ·Vol. 212 ·No. 3 ·1988-06-00 ·Pages 474-80

Lohrer H, Blum M, Herrlich P

Abstract

A subclone of an SV40-transformed fibroblast cell line from a patient with Ataxia telangiectasia (AT) with a relatively high rate of DNA uptake was isolated. However, more than 65000 independent genomic transfectants (using wild-type human DNA) did not contain the functional AT gene. This number represents the statistical distribution of an amount of DNA equivalent to more than three times the haploid human genome. The transfectants were screened by an X ray selection protocol that could rescue a single wild-type cell out of a population of 10(6) AT cells. This suggests a reversion frequency for AT of below 10(-8). The DNA uptake into human cells is compared with that into NIH3T3 cells and future possibilities for the isolation of human repair genes are discussed.

MeSH Terms
Ataxia Telangiectasia/genetics Cell Line, Transformed Cell Survival/radiation effects Cloning, Molecular DNA/genetics Fibroblasts Humans Nucleic Acid Hybridization Simian virus 40 Transfection
Chemicals
DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lohrer H
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, Federal Republic of Germany.
Blum M
Herrlich P
References (31)
31 references, click to expand
  1. Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells.
    Mol Cell Biol. 1985 May;5(5):1136-42 PMID: 3158804
  2. High mutation frequency in DNA transfected into mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3015-9 PMID: 6574469
  3. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.
    J Virol. 1979 Aug;31(2):360-9 PMID: 225559
  4. Altering genotype and phenotype by DNA-mediated gene transfer.
    Science. 1980 Sep 19;209(4463):1414-22 PMID: 7414320
  5. How damaged is the biologically active subpopulation of transfected DNA?
    Mol Cell Biol. 1984 Mar;4(3):387-98 PMID: 6325874
  6. Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli.
    Mol Cell Biol. 1984 Oct;4(10):2227-30 PMID: 6095049
  7. Studies on gene transfer and reversion to UV resistance in xeroderma pigmentosum cells.
    Somat Cell Mol Genet. 1985 Nov;11(6):617-24 PMID: 3000003
  8. DNA-mediated transfer of a human DNA repair gene that controls sister chromatid exchange.
    Mol Cell Biol. 1985 Apr;5(4):881-4 PMID: 3990694
  9. Isolation of UV-resistant revertants from a xeroderma pigmentosum complementation group A cell line.
    Nature. 1984 Sep 27-Oct 3;311(5984):390-2 PMID: 6090936
  10. Differences between rodent and human cell lines in the amount of integrated DNA after transfection.
    Exp Cell Res. 1987 Mar;169(1):111-9 PMID: 3028842
  11. Human disorders showing increased sensitivity to the induction of genetic damage.
    Annu Rev Genet. 1978;12:95-115 PMID: 371535
  12. Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4 PMID: 6304690
  13. Biological and biochemical characterization of an SV40-transformed xeroderma pigmentosum cell line.
    Exp Cell Res. 1984 Apr;151(2):408-20 PMID: 6323201
  14. Transfer of human genes conferring resistance to methylating mutagens, but not to UV irradiation and cross-linking agents, into Chinese hamster ovary cells.
    Mol Cell Biol. 1987 May;7(5):2024-30 PMID: 3600657
  15. Activation of an enhancerless gene by chromosomal integration.
    Mol Cell Biol. 1986 Dec;6(12):4179-84 PMID: 3025642
  16. DNA-mediated cotransfer of excision repair capacity and drug resistance into chinese hamster ovary mutant cell line UV-135.
    Mol Cell Biol. 1984 Jun;4(6):1152-8 PMID: 6738535
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  18. Phage particle-mediated gene transfer to cultured mammalian cells.
    Mol Cell Biol. 1982 Jun;2(6):607-16 PMID: 14582156
  19. DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.
    Mol Cell Biol. 1985 Nov;5(11):3293-6 PMID: 3018513
  20. Patterns of integration of exogenous DNA sequences transfected into mammalian cells of primate and rodent origin.
    Gene. 1986;50(1-3):279-88 PMID: 3034733
  21. Use of recombinant DNA techniques in cloning DNA repair genes and in the study of mutagenesis in mammalian cells.
    Mutat Res. 1985 Jun-Jul;150(1-2):61-7 PMID: 3889621
  22. High efficiency DNA-mediated transformation of primate cells.
    Science. 1983 Aug 5;221(4610):551-3 PMID: 6306768
  23. New procedure for DNA transfection with polycation and dimethyl sulfoxide.
    Mol Cell Biol. 1984 Jun;4(6):1172-4 PMID: 6330534
  24. High spontaneous mutation frequency in shuttle vector sequences recovered from mammalian cellular DNA.
    Mol Cell Biol. 1984 Nov;4(11):2266-72 PMID: 6096690
  25. Isolation of a transforming sequence from a human bladder carcinoma cell line.
    Cell. 1982 May;29(1):161-9 PMID: 6286138
  26. Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells.
    Nature. 1982 Apr 1;296(5856):404-9 PMID: 7063039
  27. Molecular cloning of a human DNA repair gene.
    Nature. 1984 Aug 2-8;310(5976):425-9 PMID: 6462228
  28. Ataxia telangiectasia: an inherited human disorder involving hypersensitivity to ionizing radiation and related DNA-damaging chemicals.
    Annu Rev Genet. 1979;13:291-318 PMID: 395894
  29. Transfected DNA is mutated in monkey, mouse, and human cells.
    Mol Cell Biol. 1984 Oct;4(10):1951-60 PMID: 6095032
  30. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  31. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330852
Published
1988-06-00
Pages
474-80
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330852
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2