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PMID: 6095032 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transfected DNA is mutated in monkey, mouse, and human cells.

Molecular and cellular biology ·Vol. 4 ·No. 10 ·1984-10-00 ·Pages 1951-60

Lebkowski JS, DuBridge RB, Antell EA, Greisen KS, Calos MP

Abstract

Papovavirus-based shuttle vectors containing the bacterial lacI gene were used to show that a mutation frequency in the range of 1% occurs in lacI when such vectors are transfected into COS7 and CV-1 simian cells, NIH 3T3, 3T6, L, and C127 mouse cells, and human 293 and HeLa cells. This frequency is approximately four orders of magnitude higher than the spontaneous mutation frequency in either mammalian or bacterial cells. The mutations are predominantly base substitutions and deletions and also include insertions from the mammalian genome. Time course experiments argue that mutagenesis occurs soon after arrival of the DNA into the nucleus. However, replication of the vector is not required since mutations occur even when the vector lacks all viral sequences. The high mutation frequency appears to be the characteristic outcome of transfection of DNA into mammalian cells.

MeSH Terms
Animals Cell Division Chromatography, Ion Exchange DNA Restriction Enzymes/metabolism DNA Transposable Elements DNA, Viral/genetics Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific Haplorhini Humans Kinetics Mice Mutation Plasmids Simian virus 40/genetics Transfection
Chemicals
DNA Transposable Elements DNA, Viral DNA Restriction Enzymes Deoxyribonuclease EcoRI endodeoxyribonuclease DpnI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lebkowski J S
DuBridge R B
Antell E A
Greisen K S
Calos M P
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37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.10.1951-1960.1984
Published
1984-10-00
Pages
1951-60
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369011
Subset
IM
Grants
NCI NIH HHS · CA-09302 · United States
NCI NIH HHS · CA-33056 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.10.1951-1960.1984 PMC Full Text View journal details
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