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PMID: 6308421 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Homologous and nonhomologous recombination in monkey cells.

Molecular and cellular biology ·Vol. 3 ·No. 6 ·1983-06-00 ·Pages 1040-52

Subramani S, Berg P

Abstract

Though recombinational events are important for the proper functioning of most cells, little is known about the frequency and mechanisms of recombination in mammalian cells. We have used simian virus 40 (SV40)-pBR322 hybrid plasmids constructed in vitro as substrates to detect and quantitate intramolecular homologous and nonhomologous recombination events in cultured monkey cells. Excision of wild-type or defective SV40 DNAs by recombination from these plasmids was scored by the viral plaque assay, in either the absence or the presence of DNA from a temperature-sensitive helper virus. Several independent products of homologous and nonhomologous recombination have been isolated and characterized at the DNA sequence level. We find that neither DNA replication of the recombination substrate nor SV40 large T antigen is essential for either homologous or nonhomologous recombination involving viral or pBR322 sequences.

MeSH Terms
Animals Antigens, Viral/genetics Antigens, Viral, Tumor Base Sequence Cell Line DNA, Viral/genetics Haplorhini/genetics Helper Viruses/genetics Plasmids Recombination, Genetic Simian virus 40/genetics
Chemicals
Antigens, Viral Antigens, Viral, Tumor DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Subramani S
Berg P
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50 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.6.1040-1052.1983
Published
1983-06-00
Pages
1040-52
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368633
Subset
IM
Grants
NIA NIH HHS · 5P01 AG02908-02 · United States
NIGMS NIH HHS · GM-13235 · United States
External Links
PubMed source DOI: 10.1128/mcb.3.6.1040-1052.1983 PMC Full Text View journal details
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