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PMID: 2836367 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence of the dnaB gene of Salmonella typhimurium.

Journal of bacteriology ·Vol. 170 ·No. 6 ·1988-06-00 ·Pages 2668-75

Wong A, Kean L, Maurer R

Abstract

The dnaB gene of Escherichia coli encodes a helicase that operates at replication forks of the bacterium and certain of its bacteriophages to produce separated strands suitable for subsequent use by primase and DNA polymerase III. Here, we present the sequence of the dnaB gene of Salmonella typhimurium, a functionally interchangeable analog of the E. coli dnaB gene. The DnaB proteins of these two organisms, inferred from the DNA sequences, are identical in length and in 93% of amino acid residues. Extended portions of the DnaB proteins are also similar to two phage-encoded DNA replication proteins: the gene 4 helicase-primase of coliphage T7 and, as reported previously (H. Backhaus and J. B. Petri, Gene 32: 289-303, 1984), the gene 12 protein of Salmonella phage P22. In contrast, little similarity was found between DnaB and either the UvrD repair helicase or transcription termination factor Rho (an RNA-DNA helicase). These results identify S. typhimurium DnaB as a member of the DnaB family of proteins by structural, as well as functional, criteria and provide the basis for the eventual identification, by mutational studies, of residues in DnaB critical for its function.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/analysis,genetics Base Sequence DNA, Bacterial/analysis Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Salmonella typhimurium/genetics
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wong A
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Kean L
Maurer R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.6.2668-2675.1988
Published
1988-06-00
Pages
2668-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211186
Subset
IM
Grants
NIAID NIH HHS · AI-19942 · United States
Databases
GENBANK
J03390
External Links
PubMed source DOI: 10.1128/jb.170.6.2668-2675.1988 PMC Full Text View journal details
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