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PMID: 3316192 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence analysis of the Escherichia coli dnaE gene.

Journal of bacteriology ·Vol. 169 ·No. 12 ·1987-00-00 ·Pages 5735-44

Tomasiewicz HG, McHenry CS

Abstract

We have determined the sequence of a 4,350-nucleotide region of the Escherichia coli chromosome that contains dnaE, the structural gene for the alpha subunit of DNA polymerase III holoenzyme. The dnaE gene appeared to be part of an operon containing at least three other genes: 5'-lpxB-ORF23-dnaE-ORF37-3' (ORF, open reading frame). The lpxB gene encodes lipid A disaccharide synthase, an enzyme essential for cell growth and division (M. Nishijima, C.E. Bulawa, and C.R.H. Raetz, J. Bacteriol. 145:113-121, 1981). The termination codons of lpxB and ORF23 overlapped the initiation codons of ORF23 and dnaE, respectively, suggesting translational coupling. No rho-independent transcription termination sequences were observed. A potential internal transcriptional promoter was found preceding dnaE. Deletion of the -35 region of this promoter abolished dnaE expression in plasmids lacking additional upstream sequences. From the deduced amino acid sequence, alpha had a molecular weight of 129,920 and an isoelectric point of 4.93 for the denatured protein. ORF23 encoded a more basic protein (pI 7.11) with a molecular weight of 23,228. In the accompanying paper (D.N. Crowell, W.S. Reznikoff, and C.R.H. Raetz, J. Bacteriol. 169:5727-5734, 1987), the sequence of the upstream region that contains lpxA and lpxB is reported.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Polymerase III/genetics DNA Replication DNA, Bacterial/genetics DNA-Directed DNA Polymerase/genetics Escherichia coli/genetics Genes Genes, Bacterial Molecular Sequence Data Operon Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Bacterial DNA Polymerase III DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tomasiewicz H G
Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262.
McHenry C S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.12.5735-5744.1987
Published
1987-00-00
Pages
5735-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214094
Subset
IM
Grants
NIGMS NIH HHS · GM-36255 · United States
Databases
GENBANK
M18265, M18266, M19334
Corrections
ErratumIn
PMID: 2066347
External Links
PubMed source DOI: 10.1128/jb.169.12.5735-5744.1987 PMC Full Text View journal details
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