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PMID: 6206376 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Promotion, termination, and anti-termination in the rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli K-12.

Molecular & general genetics : MGG ·Vol. 195 ·No. 3 ·1984-00-00 ·Pages 391-401

Lupski JR, Ruiz AA, Godson GN

Abstract

The regulatory regions for the rpsU-dnaG-rpoD macromolecular synthesis operon have been fused to a structural gene whose product is readily assayed (the Cmr structural gene coding for chloramphenicol acetyl transferase, CAT). The promoters (P1, P2, P3, Pa, Pb, Phs) for the macromolecular synthesis operon have different strengths as shown by their relative abilities to drive expression of the CAT gene. Promoter occlusion by P1 can be demonstrated within this operon. Regions 5kb upstream have a profound effect on operon gene expression. There is a thermoinducible promoter located within the dnaG structural gene. One of the macromolecular synthesis operon promoters is under lexA control. Although the operon structure allows coordinate expression of rpsU, dnaG and rpoD these additional features suggest that expression of individual genes can be independently regulated in response to altered growth conditions.

MeSH Terms
Acetyltransferases/genetics Chloramphenicol O-Acetyltransferase DNA Replication Escherichia coli/genetics,metabolism Gene Expression Regulation Genes Genes, Bacterial Genes, Regulator Operon Protein Biosynthesis RNA, Bacterial/biosynthesis,genetics Transcription, Genetic
Chemicals
RNA, Bacterial Acetyltransferases Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lupski J R
Ruiz A A
Godson G N
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48 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00341439
Published
1984-00-00
Pages
391-401
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
PHS HHS · 7-1142-996 · United States
Databases
GENBANK
M16194, X00773
External Links
PubMed source DOI: 10.1007/BF00341439
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