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PMID: 6304634 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The nucleotide sequence of the rho gene of E. coli K-12.

Nucleic acids research ·Vol. 11 ·No. 11 ·1983-06-11 ·Pages 3531-45

Pinkham JL, Platt T

Abstract

We have determined the nucleotide sequence of the rho gene which encodes the E. coli K-12 transcription termination factor. The structural gene was located on a cloned 3.6 kilobase BglII-HindIII restriction fragment by the introduction of the insertion element gamma delta and analysis of the recombinant plasmids by restriction analysis and in maxicells. The coding region consists of 1260 nucleotides directing the synthesis of a polypeptide 419 amino acids in length with a calculated molecular weight of 46,094. The deduced amino acid composition, amino-terminal protein sequence and calculated molecular weight are consistent with the data from the analysis of purified rho protein (16). We have shown that the rho genes from E. coli K-12, B and C strains are located on PvuII-HindIII fragments of the same size by hybridization to the rho (K-12) coding sequences.

MeSH Terms
Amino Acid Sequence Base Sequence Coliphages/genetics DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Plasmids Protein Conformation Rho Factor/genetics Transcription Factors/genetics
Chemicals
Rho Factor Transcription Factors DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pinkham J L
Platt T
References (38)
38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.11.3531
Published
1983-06-11
Pages
3531-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325984
Subset
IM
Grants
NIGMS NIH HHS · GM 22830 · United States
Databases
GENBANK
J01673, J01674
External Links
PubMed source DOI: 10.1093/nar/11.11.3531 PMC Full Text View journal details
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