Isolation and characterization of conditional-lethal rho mutants of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 3 ·1977-03-00 ·Pages 1162-6

Inoko H, Shigesada K, Imai M

Abstract

Temperature-sensitive nitA (rho) mutants of E. coli were isolated; one of them was characterized as an amber mutant. These strains show the Nit phenotype (transcription of phage lambda DNA independent of the N gene) at low temperatures and are inviable at high temperatures. The mutated sites appear to be between cya and metE on the chromosome. Temperature-sensitive nitA bacteria not only permit leftward transcription of the lambda genome at a high rate in the absence of the lambda N protein, but also allow lambda growth at low temperatures. At high temperatures, phages lambda and T4 are incapable of normal development in these cells, while growth of T7 is not affected. The production of thermally unstable rho by the nitA temperature-sensitive mutant suggests that nitA is the structural gene for rho.

MeSH Terms

Anthranilate Synthase/biosynthesis Coliphages/growth & development DNA, Viral/metabolism Escherichia coli/metabolism Genes Mutation RNA, Viral/biosynthesis Rho Factor/biosynthesis,physiology Temperature Transcription Factors/biosynthesis Transcription, Genetic

Chemicals

DNA, Viral RNA, Viral Rho Factor Transcription Factors Anthranilate Synthase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Inoko H

Shigesada K

Imai M

References (27)

27 references, click to expand

Recalibrated linkage map of Escherichia coli K-12.
Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
Mutations affecting growth of the Escherichia coli cell under a condition of DNA polymerase I-deficiency.
Mol Gen Genet. 1973;123(1):89-110 PMID: 4353650
Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1959-63 PMID: 132662
A simple procedure for localized mutagenesis using nitrosoguanidine.
Mol Gen Genet. 1974;134(1):77-83 PMID: 4617157
Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12.
Mol Gen Genet. 1973;123(1):77-88 PMID: 4580085
Control of gene expression in bacteriophage lambda.
Annu Rev Genet. 1973;7:289-324 PMID: 4593306
Interference with the expression of the N gene function of phage lambda in a mutant of Escherichia coli.
Virology. 1973 Jan;51(1):216-26 PMID: 4567743
Regulation of the tryptophan operon of Escherichia coli integrated into the phage phi 80 genome.
Mol Gen Genet. 1974 Mar 6;129(1):37-48 PMID: 4600013
Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2534-8 PMID: 4601822
Diversity of regulation of genetic transcription. II. Specific relaxation of polarity in read-through transcription of the translocated trp operon in bacteriophage lambda trp.
J Mol Biol. 1974 Aug 25;87(4):741-54 PMID: 4427375
Relationship between the N function of bacteriophage lambda and host RNA polymerase.
J Mol Biol. 1972 Mar 28;65(2):259-72 PMID: 5040359
Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase.
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2977-81 PMID: 4943550
Regulation of bacteriophage lambda development by gene N: properties of a mutation that bypasses N control of late protein synthesis.
Virology. 1970 Feb;40(2):212-22 PMID: 4909409
Termination factor for RNA synthesis.
Nature. 1969 Dec 20;224(5225):1168-74 PMID: 4902144
Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations.
Proc Natl Acad Sci U S A. 1975 May;72(5):1725-8 PMID: 1098042
Isolation and genetic characterization of the nitA mutants of Escherichia coli affecting the termination factor rho.
Mol Gen Genet. 1976 Jan 16;143(2):211-21 PMID: 765755
Evidence that mutations in the suA polarity suppressing gene directly affect termination factor rho.
Nature. 1976 Jan 15;259(5539):151-3 PMID: 1107855
Polarity suppressors increase expression of the wild-type tryptophan operon of Escherichia coli.
J Mol Biol. 1976 May 15;103(2):395-409 PMID: 781273
Bacterial mutants able to partly suppress the effect of N mutations in bacteriophage lambda.
Mol Gen Genet. 1975;136(2):167-80 PMID: 16094982
Localization of P2 prophage in two strains of Escherichia coli.
Virology. 1963 Jan;19:32-9 PMID: 14042138
Lysogeny.
Adv Virus Res. 1958;5:151-93 PMID: 13508404
Polarity suppressors defective in transcription termination at the attenuator of the tryptophan operon of Escherichia coli have altered rho factor.
J Mol Biol. 1976 Sep 15;106(2):231-41 PMID: 135845
Relief of polarity in E. coli by "suA".
Nature. 1970 Apr 4;226(5240):28-31 PMID: 4907561
An RNA-dependent nucleoside triphosphate phosphohydrolase (ATPase) associated with rho termination factor.
Proc Natl Acad Sci U S A. 1974 May;71(5):2003-7 PMID: 4365581
Altered reading of genetic signals fused to the N operon of bacteriophage lambda: genetic evidence for modification of polymerase by the protein product of the N gene.
J Mol Biol. 1974 Oct 15;89(1):33-48 PMID: 4613856
In vitro transcription of the tryptophan operon integrated into a transducing phage genome.
J Mol Biol. 1974 Apr 5;84(2):315-35 PMID: 4830867
Effect of Rho on transcription of bacterial operons.
Nat New Biol. 1973 Feb 28;241(113):260-4 PMID: 4348358

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.3.1162

Published

1977-03-00

Pages

1162-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430633

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.3.1162 PMC Full Text View journal details

Analysis Services

Isolation and characterization of conditional-lethal rho mutants of Escherichia coli.

Contact

WeChat Official Account