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PMID: 2825164 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Capsid protein VP4 of poliovirus is N-myristoylated.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 22 ·1987-11-00 ·Pages 7827-31

Paul AV, Schultz A, Pincus SE, Oroszlan S, Wimmer E

Abstract

Poliovirus was labeled in vivo with [3H]myristic acid. Analysis of the capsid polypeptides revealed that the [3H]myristic acid residues copurified with VP4, the smallest and internal capsid protein of the virion. Evidence is presented showing unambiguously that the N-terminal glycine residue of VP4 is N-myristoylated. A previous analysis of the tryptic peptides of VP4 [Dorner, A. J., Dorner, L. F., Larsen, G. R., Wimmer, E. & Anderson, C. W. (1982) J. Virol. 42, 1017-1028] had shown that the N-terminal blocking group exists on all VP4 molecules as well as on VP0 and P1, two precursor polypeptides to VP4 in poliovirus. The possible function of the myristic acid residue in VP4 and in its precursor in poliovirus proliferation is discussed.

MeSH Terms
Capsid/analysis,biosynthesis Capsid Proteins Glycine/analysis Myristic Acid Myristic Acids/analysis Poliovirus/analysis,physiology Protein Precursors/metabolism Protein Processing, Post-Translational Virus Replication
Chemicals
Capsid Proteins Myristic Acids Protein Precursors VP1 protein, Poliovirus Myristic Acid Glycine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Paul A V
Department of Microbiology, School of Medicine, State University of New York at Stony Brook 11794.
Schultz A
Pincus S E
Oroszlan S
Wimmer E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.22.7827
Published
1987-11-00
Pages
7827-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299409
Subset
IM
Grants
NIAID NIH HHS · AI15122 · United States
NCI NIH HHS · CA28146 · United States
NCI NIH HHS · N01-CO-23909 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.22.7827 PMC Full Text View journal details
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