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PMID: 6314267 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the 5' noncoding region and part of the gag gene of mouse mammary tumor virus; identification of the 5' splicing site for subgenomic mRNAs.

Nucleic acids research ·Vol. 11 ·No. 20 ·1983-10-25 ·Pages 6943-55

Fasel N, Buetti E, Firzlaff J, Pearson K, Diggelmann H

Abstract

We have determined the sequence of the first 1371 nucleotides at the 5' end of the genome of mouse mammary tumor virus using molecularly cloned proviral DNA of the GR virus strain. The most likely initiation codon used for the gag gene of mouse mammary tumor virus is the first one, located 312 nucleotides from the 5' end of the viral RNA. The 5' splicing site for the subgenomic mRNA's is located approximately 288 nucleotides downstream from the 5' end of the viral RNA. From the DNA sequence the amino acid sequence of the N-terminal half of the gag precursor protein, including p10 and p21, was deduced (353 amino acids).

MeSH Terms
Amino Acid Sequence Animals Antigens, Viral/genetics Base Sequence Cloning, Molecular Codon/genetics Coliphages/genetics DNA/analysis DNA Restriction Enzymes Gene Products, gag Genes Genes, Viral Mammary Tumor Virus, Mouse/genetics,immunology RNA, Messenger/genetics Viral Proteins/genetics
Chemicals
Antigens, Viral Codon Gene Products, gag RNA, Messenger Viral Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fasel N
Buetti E
Firzlaff J
Pearson K
Diggelmann H
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.20.6943
Published
1983-10-25
Pages
6943-55
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326430
Subset
IM
Databases
GENBANK
X00018
External Links
PubMed source DOI: 10.1093/nar/11.20.6943 PMC Full Text View journal details
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