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PMID: 2988939 Published · ppublish English Journal Article

Two classes of fatty acid acylated proteins exist in eukaryotic cells.

The EMBO journal ·Vol. 4 ·No. 5 ·1985-05-00 ·Pages 1137-44

Magee AI, Courtneidge SA

Abstract

Labelling of cultured cells with [3H]palmitic and [3H]myristic acids demonstrates that each of these fatty acids modifies a substantially different subset of cellular proteins. Hydroxylamine treatment can be used to differentiate sensitive thioester linkages to palmitate from insensitive amide linkages to myristate. Several palmitoylated proteins are surface-oriented glycoproteins while all of the myristylated proteins appear to be internal. Myristate addition is much more tightly coupled to protein synthesis than palmitoylation, which is able to continue at a reduced level even in the prolonged absence of protein synthesis. Acyl proteins patterns were affected both qualitatively and quantitatively by transformation and growth status. The preferential addition of palmitate to the transferrin receptor and myristate to pp60src, and the absence of these modifications from several other proteins is reported. We propose a nomenclature for fatty acyl proteins based on these observations.

MeSH Terms
Acylation Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/analysis Cattle Cell Transformation, Neoplastic/metabolism Cells, Cultured Chick Embryo Cricetinae Dogs Emetine/pharmacology Fatty Acids/metabolism Humans Myristic Acid Myristic Acids/metabolism Palmitic Acid Palmitic Acids/metabolism Proteins/metabolism Rats Receptors, Cell Surface/analysis Receptors, Transferrin Tunicamycin/pharmacology Viral Proteins/analysis
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Fatty Acids Myristic Acids Palmitic Acids Proteins Receptors, Cell Surface Receptors, Transferrin Viral Proteins Myristic Acid Tunicamycin Palmitic Acid Emetine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Magee A I
Courtneidge S A
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43 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-05-00
Pages
1137-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554315
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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