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PMID: 2820872 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evolution of pseudogenes in the immunoglobulin VH-gene family of the mouse.

Immunogenetics ·Vol. 26 ·No. 4-5 ·1987-00-00 ·Pages 237-48

Blankenstein T, Bonhomme F, Krawinkel U

Abstract

A quantitative analysis of the complexity of the J558 VH-gene family in the mouse immunoglobulin heavy chain (Igh) gene locus has been performed. Considerable variations in the degree of complexity are observed in various Igh haplotypes derived from laboratory mice and wild mice. The BALB/c strain shows the highest degree of complexity of the J558 VH-gene family when all mice are compared. Multiple gene duplications seem to have occurred in the BALB/c-derived J558 VH-gene family less than 1-2 million years ago. This dating is supported by the divergence in coding and flanking regions of three strongly homologous VH-region genes. Two of these genes were generated by the duplication of a pseudogene about 1.5 X 10(5) years ago. A recent expansion of the J558 VH-gene family and therefore little time for evolutionary drift may explain why most of the pseudogenes in this family exhibit a largely intact structure. We also describe two VH-region genes which represent older pseudogenes in states of progressive disintegration.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cloning, Molecular DNA Restriction Enzymes Genes, Immunoglobulin Immunoglobulin Variable Region/genetics Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Molecular Sequence Data Nucleic Acid Hybridization Pseudogenes Rats Rats, Inbred Strains
Chemicals
Immunoglobulin Variable Region DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blankenstein T
Institut für Genetik der Universität zu Köln, Federal Republic of Germany.
Bonhomme F
Krawinkel U
References (36)
36 references, click to expand
  1. Attempts to locate complementarity-determining residues in the variable positions of light and heavy chains.
    Ann N Y Acad Sci. 1971 Dec 31;190:382-93 PMID: 5290024
  2. Variability of evolutionary rates of DNA.
    Genetics. 1986 Aug;113(4):1077-91 PMID: 3744027
  3. Recombination between antibody heavy chain variable-region genes: evidence for gene conversion.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4997-5001 PMID: 6308665
  4. The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes.
    Eur J Immunol. 1984 Oct;14(10):922-30 PMID: 6092095
  5. The potential use of repetitive sequences in phylogenetic reconstructions.
    Curr Top Microbiol Immunol. 1986;127:45-52 PMID: 3731854
  6. A hybrid origin of Japanese mice "Mus musculus molossinus".
    Curr Top Microbiol Immunol. 1986;127:62-7 PMID: 3731855
  7. Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family.
    Nucleic Acids Res. 1984 Sep 11;12(17):6887-900 PMID: 6091043
  8. Mitochondrial DNA evolution in mice.
    Genetics. 1983 Nov;105(3):681-721 PMID: 6315529
  9. Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3630-4 PMID: 6251474
  10. Biochemical diversity and evolution in the genus Mus.
    Biochem Genet. 1984 Apr;22(3-4):275-303 PMID: 6375655
  11. Diversity of germ-line immunoglobulin VH genes.
    Nature. 1981 Jul 30;292(5822):426-30 PMID: 6789211
  12. Evolutionary relationships in the genus Mus.
    Curr Top Microbiol Immunol. 1986;127:19-34 PMID: 3731841
  13. Gene conversion: some implications for immunoglobulin genes.
    Cell. 1981 Jun;24(3):592-4 PMID: 7018693
  14. Immunoglobulin V region variants in hybridoma cells. I. Isolation of a variant with altered idiotypic and antigen binding specificity.
    EMBO J. 1982;1(5):629-34 PMID: 6765233
  15. Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes.
    EMBO J. 1985 Nov;4(11):2861-7 PMID: 2998759
  16. DNA-DNA hybridization evidence of the rapid rate of muroid rodent DNA evolution.
    Mol Biol Evol. 1987 May;4(3):242-53 PMID: 3447010
  17. Origin and evolution of mice: an appraisal of fossil evidence and morphological traits.
    Curr Top Microbiol Immunol. 1986;127:3-11 PMID: 3731849
  18. One heavy chain variable region gene segment subfamily in the BALB/c mouse contains 500-1000 or more members.
    Cell. 1986 Nov 7;47(3):461-70 PMID: 3094964
  19. Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell lines.
    Nature. 1984 Oct 25-31;311(5988):727-33 PMID: 6092962
  20. Simple DNA sequences in homologous flanking regions near immunoglobulin VH genes: a role in gene interaction?
    Nucleic Acids Res. 1982 Jun 11;10(11):3353-70 PMID: 6285297
  21. Deletion of Cmu genes in mouse B lymphocytes upon stimulation with LPS.
    EMBO J. 1983;2(11):1929-35 PMID: 6315407
  22. Evolutionary relationships among five subspecies of Mus musculus based on restriction enzyme cleavage patterns of mitochondrial DNA.
    Genetics. 1981 Aug;98(4):801-16 PMID: 6277733
  23. Allelic immunoglobulin VH genes in two mouse strains: possible germline gene recombination.
    EMBO J. 1983;2(11):2013-8 PMID: 6416826
  24. The genetic control of immunoglobulin synthesis.
    Annu Rev Genet. 1972;6:1-46 PMID: 4124595
  25. Evolutionary rate at the molecular level.
    Nature. 1968 Feb 17;217(5129):624-6 PMID: 5637732
  26. Functional and pseudogenes are similarly organized and may equally contribute to the extensive antibody diversity of the IgVHII family.
    EMBO J. 1985 May;4(5):1225-30 PMID: 3924600
  27. Conservation and divergence of immunoglobulin VH pseudogenes.
    EMBO J. 1983;2(10):1795-800 PMID: 6315403
  28. Drastic change in idiotypic but not antigen-binding specificity of an antibody by a single amino-acid substitution.
    Nature. 1985 Jun 6-12;315(6019):506-8 PMID: 2582273
  29. An immunoglobulin VH pseudogene.
    J Mol Appl Genet. 1981;1(2):93-101 PMID: 6286821
  30. VH-gene expression in murine lipopolysaccharide blasts distributes over the nine known VH-gene groups and may be random.
    Eur J Immunol. 1985 Nov;15(11):1154-6 PMID: 3933987
  31. A new classification of mouse VH sequences.
    Immunol Today. 1984 Apr;5(4):85-6 PMID: 25291694
  32. Molecular basis of a mouse strain-specific anti-hapten response.
    Cell. 1983 May;33(1):85-93 PMID: 6432337
  33. Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.
    Cell. 1981 Jun;24(3):625-37 PMID: 6788376
  34. A hyperconversion mechanism generates the chicken light chain preimmune repertoire.
    Cell. 1987 Feb 13;48(3):379-88 PMID: 3100050
  35. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  36. Idiotypic selection of an antibody mutant with changed hapten binding specificity, resulting from a point mutation in position 50 of the heavy chain.
    EMBO J. 1986 Jul;5(7):1561-6 PMID: 3017703
Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00346518
Published
1987-00-00
Pages
237-48
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Databases
GENBANK
M17570, M17571, M17572, M17573, M17574, M17575, M17576, M17577, M17578, M17579, M17580, M17581, M17696, M19945, X06856, X06857, X06858, X06860, X06862, X06863, X06864, X06865, X06866, X06867, X14516, X14517
External Links
PubMed source DOI: 10.1007/BF00346518 View journal details
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