Home LiteratureArticle Details
PMID: 2998759 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes.

The EMBO journal ·Vol. 4 ·No. 11 ·1985-11-00 ·Pages 2861-7

Winter E, Radbruch A, Krawinkel U

Abstract

Four potentially productive and two non-productive VDJ gene segments were isolated from the DNA of mouse B-lymphocytes which had been polyclonally activated by bacterial lipopolysaccharide (LPS). Three VDJ regions exhibit VH genes which stem from two novel VH gene families. The complexity of these families is 5-9 genes. One of the non-productive VDJ regions exhibits a D segment which may have been generated by joining of two DSP2 segments. Both non-productive VDJ regions appear to contain rearranged pseudo VH genes. Three potential somatic mutations distributed over two productive VDJ regions are observed.

MeSH Terms
Animals B-Lymphocytes/immunology Bacteriophage lambda/genetics Base Sequence DNA/isolation & purification DNA Restriction Enzymes Genes Genetic Variation Immunoglobulin Variable Region/genetics Liver/immunology Lymphocyte Activation Mice Mice, Inbred BALB C Mice, Inbred C57BL Nucleic Acid Hybridization
Chemicals
Immunoglobulin Variable Region DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Winter E
Radbruch A
Krawinkel U
References (28)
28 references, click to expand
  1. Molecular basis of an isogeneic anti-idiotypic response.
    EMBO J. 1984 Dec 1;3(12):3005-12 PMID: 6084592
  2. The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes.
    Eur J Immunol. 1984 Oct;14(10):922-30 PMID: 6092095
  3. Clonal growth and maturation to immunoglobulin secretion in vitro of every growth-inducible B lymphocyte.
    Cell. 1977 Jan;10(1):27-34 PMID: 319912
  4. Model-building studies of antigen-binding sites: the hapten-binding site of mopc-315.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 2:627-37 PMID: 268248
  5. Preliminary refinement and structural analysis of the Fab fragment from human immunoglobulin new at 2.0 A resolution.
    J Biol Chem. 1978 Jan 25;253(2):585-97 PMID: 618887
  6. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  7. Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):509-13 PMID: 6787590
  8. Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.
    Cell. 1981 Jun;24(3):625-37 PMID: 6788376
  9. Diversity of germ-line immunoglobulin VH genes.
    Nature. 1981 Jul 30;292(5822):426-30 PMID: 6789211
  10. Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments.
    J Exp Med. 1982 Jan 1;155(1):201-18 PMID: 6798155
  11. A history of the human fetal globin gene duplication.
    Cell. 1981 Oct;26(2 Pt 2):191-203 PMID: 7332928
  12. Simple DNA sequences in homologous flanking regions near immunoglobulin VH genes: a role in gene interaction?
    Nucleic Acids Res. 1982 Jun 11;10(11):3353-70 PMID: 6285297
  13. Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4118-22 PMID: 6287467
  14. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  15. The genetic basis of antibody production: a single heavy chain variable region gene encodes all molecules bearing the dominant anti-arsonate idiotype in the strain A mouse.
    Eur J Immunol. 1983 Feb;13(2):123-32 PMID: 6403356
  16. Somatic generation of antibody diversity.
    Nature. 1983 Apr 14;302(5909):575-81 PMID: 6300689
  17. Recombination between antibody heavy chain variable-region genes: evidence for gene conversion.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4997-5001 PMID: 6308665
  18. Conservation and divergence of immunoglobulin VH pseudogenes.
    EMBO J. 1983;2(10):1795-800 PMID: 6315403
  19. Deletion of Cmu genes in mouse B lymphocytes upon stimulation with LPS.
    EMBO J. 1983;2(11):1929-35 PMID: 6315407
  20. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  21. Somatic mutation creates diversity in the major group of mouse immunoglobulin kappa light chains.
    J Exp Med. 1984 Feb 1;159(2):417-35 PMID: 6420501
  22. Expression and rearrangement of homologous immunoglobulin VH genes in two mouse strains.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2167-71 PMID: 6326109
  23. The human T cell antigen receptor is encoded by variable, diversity, and joining gene segments that rearrange to generate a complete V gene.
    Cell. 1984 Jun;37(2):393-401 PMID: 6202421
  24. Ordered rearrangement of immunoglobulin heavy chain variable region segments.
    EMBO J. 1984 Jun;3(6):1209-19 PMID: 6086308
  25. Molecular basis of a mouse strain-specific anti-hapten response.
    Cell. 1983 May;33(1):85-93 PMID: 6432337
  26. The structure, rearrangement and expression of D beta gene segments of the murine T-cell antigen receptor.
    Nature. 1984 Sep 27-Oct 3;311(5984):344-50 PMID: 6090932
  27. Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family.
    Nucleic Acids Res. 1984 Sep 11;12(17):6887-900 PMID: 6091043
  28. Structural basis of antibody function.
    Annu Rev Immunol. 1983;1:87-117 PMID: 6399980
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-11-00
Pages
2861-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554590
Subset
IM
Databases
GENBANK
X03087, X03088, X03089, X03090, X03091, X03300, X03301, X03302, X03303
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2