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PMID: 3017703 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Idiotypic selection of an antibody mutant with changed hapten binding specificity, resulting from a point mutation in position 50 of the heavy chain.

The EMBO journal ·Vol. 5 ·No. 7 ·1986-07-00 ·Pages 1561-6

Brüggemann M, Müller HJ, Burger C, Rajewsky K

Abstract

Somatic mutation occurs at a low rate in the rearranged antibody V region genes of the hybridoma line B1-8. delta 1 which expresses an antibody with specificity for the hapten 4-hydroxy-3-nitro-5-iodo-phenylacetyl (NIP). A mutant was selected which had lost a binding site-related idiotope but retained most of its other idiotypic determinants. The mutant had concomitantly lost NIP binding and acquired specificity for dinitrophenylated bovine serum albumin. It carried a single point mutation in position 50 of the heavy chain, resulting in the replacement of an arginine by a glycine.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Base Sequence Cell Line DNA Restriction Enzymes Fluorescent Antibody Technique Haptens Hybridomas/immunology Immunoglobulin Heavy Chains/genetics Immunoglobulin Idiotypes/genetics,immunology Immunoglobulin Variable Region/genetics Mice Mutation Plasmacytoma
Chemicals
Antibodies, Monoclonal Haptens Immunoglobulin Heavy Chains Immunoglobulin Idiotypes Immunoglobulin Variable Region DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brüggemann M
Müller H J
Burger C
Rajewsky K
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41 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-07-00
Pages
1561-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166980
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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