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PMID: 6803237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Somatic mutation in a cultured mouse myeloma cell affects antigen binding.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 4 ·1982-02-00 ·Pages 1240-4

Cook WD, Rudikoff S, Giusti AM, Scharff MD

Abstract

The S107 mouse myeloma cell line synthesizes an IgA antibody that binds the hapten phosphocholine and is similar if not identical in its heavy and light chain variable region sequence to the predominant antibody produced by BALB/c mice in response to immunization with phosphocholine. This cell line frequently and spontaneously generates somatic variants producing immunoglobulins with decreased ability to bind antigen. One such variant, S 107.U1, is described here. This variant has a decreased ability to bind phosphocholine when it is attached to a carrier, although its affinity for free hapten is the same as that of the parent. This decrease in antigen binding is associated with a single amino acid substitution at the fifth residue in the JH segment.

MeSH Terms

Amino Acid Sequence Animals Antigen-Antibody Reactions Binding Sites, Antibody/genetics Cell Line Haptens/immunology Immunoglobulin A/genetics,immunology Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Mice Multiple Myeloma Mutation Phosphorylcholine/immunology

Chemicals

Haptens Immunoglobulin A Immunoglobulin Heavy Chains Immunoglobulin Variable Region Phosphorylcholine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Cook W D

Rudikoff S

Giusti A M

Scharff M D

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.4.1240

Published

1982-02-00

Pages

1240-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC345937

Subset

Grants

NCI NIH HHS · 5T32-CA09173-06 · United States

NIAID NIH HHS · AI 10702 · United States

NIAID NIH HHS · AI 5231 · United States

External Links

PubMed source DOI: 10.1073/pnas.79.4.1240 PMC Full Text View journal details

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Somatic mutation in a cultured mouse myeloma cell affects antigen binding.

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