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PMID: 27911188 Published · ppublish English Journal Article Review Research Support, Non-U.S. Gov't

Tuning the ribosome: The influence of rRNA modification on eukaryotic ribosome biogenesis and function.

RNA biology ·Vol. 14 ·No. 9 ·2017-00-02 ·Pages 1138-1152

Sloan KE, Warda AS, Sharma S, Entian KD, Lafontaine DLJ, Bohnsack MT

Abstract

rRNAs are extensively modified during their transcription and subsequent maturation in the nucleolus, nucleus and cytoplasm. RNA modifications, which are installed either by snoRNA-guided or by stand-alone enzymes, generally stabilize the structure of the ribosome. However, they also cluster at functionally important sites of the ribosome, such as the peptidyltransferase center and the decoding site, where they facilitate efficient and accurate protein synthesis. The recent identification of sites of substoichiometric 2'-O-methylation and pseudouridylation has overturned the notion that all rRNA modifications are constitutively present on ribosomes, highlighting nucleotide modifications as an important source of ribosomal heterogeneity. While the mechanisms regulating partial modification and the functions of specialized ribosomes are largely unknown, changes in the rRNA modification pattern have been observed in response to environmental changes, during development, and in disease. This suggests that rRNA modifications may contribute to the translational control of gene expression.

Keywords
RNA methyltransferase RNA modification acetylation methylation pseudouridylation ribosome ribosomopathy snoRNA translation
MeSH Terms
Acetylation Animals Disease Susceptibility Eukaryotic Cells/physiology Humans Methylation RNA, Ribosomal/chemistry,genetics,metabolism RNA, Small Nucleolar/genetics,metabolism Ribosomes/chemistry,metabolism Structure-Activity Relationship
Chemicals
RNA, Ribosomal RNA, Small Nucleolar
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sloan Katherine E
a Institute for Molecular Biology, University Medical Center Göttingen, Georg-August-University , Göttingen , Germany.
Warda Ahmed S
a Institute for Molecular Biology, University Medical Center Göttingen, Georg-August-University , Göttingen , Germany.
Sharma Sunny
b RNA Molecular Biology and Center for Microscopy and Molecular Imaging, F.R.S./FNRS, Université Libre de Bruxelles , Charleroi-Gosselies , Belgium.
Entian Karl-Dieter
c Institute for Molecular Biosciences, Goethe University , Frankfurt am Main , Germany.
Lafontaine Denis L J
b RNA Molecular Biology and Center for Microscopy and Molecular Imaging, F.R.S./FNRS, Université Libre de Bruxelles , Charleroi-Gosselies , Belgium.
Bohnsack Markus T
a Institute for Molecular Biology, University Medical Center Göttingen, Georg-August-University , Göttingen , Germany. | d Göttingen Centre for Molecular Biosciences, Georg-August-University , Göttingen , Germany.
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Article Info
Journal
RNA biology
Abbr.
RNA Biol
ISSN
1555-8584
DOI
10.1080/15476286.2016.1259781
Published
2017-00-02
Epub
2016-00-02
Pages
1138-1152
Language
English
Region
United States
NLM ID
101235328
PMCID
PMC5699541
Subset
IM
External Links
PubMed source DOI: 10.1080/15476286.2016.1259781 PMC Full Text View journal details
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