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PMID: 25411247 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human NAT10 is an ATP-dependent RNA acetyltransferase responsible for N4-acetylcytidine formation in 18 S ribosomal RNA (rRNA).

The Journal of biological chemistry ·Vol. 289 ·No. 52 ·2014-12-26 ·Pages 35724-30

Ito S, Horikawa S, Suzuki T, Kawauchi H, Tanaka Y, Suzuki T, Suzuki T

Abstract

Human N-acetyltransferase 10 (NAT10) is known to be a lysine acetyltransferase that targets microtubules and histones and plays an important role in cell division. NAT10 is highly expressed in malignant tumors, and is also a promising target for therapies against laminopathies and premature aging. Here we report that NAT10 is an ATP-dependent RNA acetyltransferase responsible for formation of N(4)-acetylcytidine (ac(4)C) at position 1842 in the terminal helix of mammalian 18 S rRNA. RNAi-mediated knockdown of NAT10 resulted in growth retardation of human cells, and this was accompanied by high-level accumulation of the 30 S precursor of 18 S rRNA, suggesting that ac(4)C1842 formation catalyzed by NAT10 is involved in rRNA processing and ribosome biogenesis.

Keywords
Acetyl Coenzyme A (Acetyl-CoA) Acetyltransferase RNA Modification Ribosomal RNA Processing (rRNA Processing) Ribosome Assembly
MeSH Terms
Acetylation Base Sequence HEK293 Cells HeLa Cells Humans Molecular Sequence Data N-Terminal Acetyltransferase E/physiology N-Terminal Acetyltransferases Nucleic Acid Conformation RNA Processing, Post-Transcriptional RNA, Ribosomal, 18S/metabolism
Chemicals
RNA, Ribosomal, 18S N-Terminal Acetyltransferase E N-Terminal Acetyltransferases NAT10 protein, human
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ito Satoshi
From the Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 and.
Horikawa Sayuri
From the Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 and.
Suzuki Tateki
the Graduate School of Life Science and.
Kawauchi Hiroki
the Graduate School of Life Science and.
Tanaka Yoshikazu
the Graduate School of Life Science and Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan.
Suzuki Takeo
From the Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 and.
Suzuki Tsutomu
From the Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 and ts@chembio.t.u-tokyo.ac.jp.
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.C114.602698
Email
ts@chembio.t.u-tokyo.ac.jp
Published
2014-12-26
Epub
2014-00-19
Pages
35724-30
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC4276842
Subset
IM
External Links
PubMed source DOI: 10.1074/jbc.C114.602698 PMC Full Text View journal details
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