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PMID: 21177859 Published · ppublish English Journal Article

hALP, a novel transcriptional U three protein (t-UTP), activates RNA polymerase I transcription by binding and acetylating the upstream binding factor (UBF).

The Journal of biological chemistry ·Vol. 286 ·No. 9 ·2011-03-04 ·Pages 7139-48

Kong R, Zhang L, Hu L, Peng Q, Han W, Du X, Ke Y

Abstract

Transcription of ribosome RNA precursor (pre-rRNA) and pre-rRNA processing are coordinated by a subset of U three proteins (UTPs) known as transcriptional UTPs (t-UTPs), which participate in pre-rRNA transcription in addition to participation in 18 S rRNA processing. However, the mechanism by which t-UTPs function in pre-rRNA transcription remains undetermined. In the present study, we identified hALP, a histone acetyl-transferase as a novel t-UTP. We first showed that hALP is nucleolar, and is associated with U3 snoRNA and required for 18 S rRNA processing. Moreover, depletion of hALP resulted in a decreased level of 47 S pre-rRNA. Ectopic expression of hALP activated the rDNA promoter luciferase reporter and knockdown of hALP inhibited the reporter. In addition, hALP bound rDNA. Taken together these data identify hALP as a novel t-UTP. Immunoprecipitation and GST pulldown experiments showed that hALP binds the upstream binding factor (UBF) in vivo and in vitro. It is of importance that hALP acetylated UBF depending on HAT in vivo, and hALP but not hALP (ΔHAT) facilitated the nuclear translocation of the RNA polymerase I (Pol I)-associated factor 53 (PAF53) from the cytoplasm and promoted the association of UBF with PAF53. Thus, we provide a mechanism in which a novel t-UTP activates Pol I transcription by binding and acetylating UBF.

MeSH Terms
Acetylation Active Transport, Cell Nucleus/physiology Asparaginase/genetics,metabolism Autoantigens/genetics,metabolism Cell Nucleolus/physiology Cytoplasm/metabolism HEK293 Cells Humans Plasmids/genetics Pol1 Transcription Initiation Complex Proteins/genetics,metabolism Protein Binding/physiology RNA Polymerase I/genetics,metabolism RNA, Ribosomal, 18S/genetics,metabolism RNA, Small Interfering RNA, Small Nucleolar/metabolism Transcription Factors/metabolism Transcriptional Activation/physiology
Chemicals
Autoantigens POLR1E protein, human Pol1 Transcription Initiation Complex Proteins RNA, Ribosomal, 18S RNA, Small Interfering RNA, Small Nucleolar RNA, U3 small nucleolar Transcription Factors transcription factor UBF RNA Polymerase I ASRGL1 protein, human Asparaginase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kong Ruirui
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China.
Zhang Liangliang
Hu Lelin
Peng Qunhui
Han Wei
Du Xiaojuan
Ke Yang
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M110.173393
Published
2011-03-04
Epub
2010-00-22
Pages
7139-48
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3044971
Subset
IM
External Links
PubMed source DOI: 10.1074/jbc.M110.173393 PMC Full Text View journal details
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