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PMID: 11250901 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Acetylation of TAF(I)68, a subunit of TIF-IB/SL1, activates RNA polymerase I transcription.

The EMBO journal ·Vol. 20 ·No. 6 ·2001-03-15 ·Pages 1353-62

Muth V, Nadaud S, Grummt I, Voit R

Abstract

Mammalian rRNA genes are preceded by a terminator element that is recognized by the transcription termination factor TTF-I. In exploring the functional significance of the promoter-proximal terminator, we found that TTF-I associates with the p300/CBP-associated factor PCAF, suggesting that TTF-I may target histone acetyltransferase to the rDNA promoter. We demonstrate that PCAF acetylates TAF(I)68, the second largest subunit of the TATA box-binding protein (TBP)-containing factor TIF-IB/SL1, and acetylation enhances binding of TAF(I)68 to the rDNA promoter. Moreover, PCAF stimulates RNA polymerase I (Pol I) transcription in a reconstituted in vitro system. Consistent with acetylation of TIF-IB/SL1 being required for rDNA transcription, the NAD(+)-dependent histone deacetylase mSir2a deacetylates TAF(I)68 and represses Pol I transcription. The results demonstrate that acetylation of the basal Pol I transcription machinery has functional consequences and suggest that reversible acetylation of TIF-IB/SL1 may be an effective means to regulate rDNA transcription in response to external signals.

MeSH Terms
Acetylation Acetyltransferases/antagonists & inhibitors,metabolism Animals DNA, Ribosomal DNA-Binding Proteins/metabolism Gene Expression Regulation, Enzymologic Gene Silencing Histone Acetyltransferases Histone Deacetylases/metabolism Hydroxamic Acids/pharmacology Mice Pol1 Transcription Initiation Complex Proteins Protein Binding Protein Subunits RNA Polymerase I/biosynthesis,genetics Recombinant Proteins/metabolism Saccharomyces cerevisiae Proteins Silent Information Regulator Proteins, Saccharomyces cerevisiae Sirtuin 2 Sirtuins TATA-Box Binding Protein Terminator Regions, Genetic Trans-Activators/metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
DNA, Ribosomal DNA-Binding Proteins Hydroxamic Acids Pol1 Transcription Initiation Complex Proteins Protein Subunits Recombinant Proteins Saccharomyces cerevisiae Proteins Silent Information Regulator Proteins, Saccharomyces cerevisiae TAF(i)68 protein, mouse TATA-Box Binding Protein Trans-Activators Transcription Factors transcription initiation factor TIF-IB trichostatin A Acetyltransferases Histone Acetyltransferases RNA Polymerase I SIR2 protein, S cerevisiae Sirtuin 2 Sirtuins Histone Deacetylases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Muth V
Division of Molecular Biology of the Cell II, German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
Nadaud S
Grummt I
Voit R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.6.1353
Published
2001-03-15
Pages
1353-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC145524
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.6.1353 PMC Full Text View journal details
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