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PMID: 15489292 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components.

Genes & development ·Vol. 18 ·No. 20 ·2004-10-15 ·Pages 2506-17

Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer AL, Baserga SJ

Abstract

Sequential events in macromolecular biosynthesis are often elegantly coordinated. The small ribosomal subunit (SSU) processome is a large ribonucleoprotein (RNP) required for processing of precursors to the small subunit RNA, the 18S, of the ribosome. We have found that a subcomplex of SSU processome proteins, the t-Utps, is also required for optimal rRNA transcription in vivo in the yeast Saccharomyces cerevisiae. The t-Utps are ribosomal chromatin (r-chromatin)-associated, and they exist in a complex in the absence of the U3 snoRNA. Transcription is required neither for the formation of the subcomplex nor for its r-chromatin association. The t-Utps are associated with the pre-18S rRNAs independent of the presence of the U3 snoRNA. This association may thus represent an early step in the formation of the SSU processome. Our results indicate that rRNA transcription and pre-rRNA processing are coordinated via specific components of the SSU processome.

MeSH Terms
Blotting, Western Chromatin/chemistry DNA Primers Immunoprecipitation Models, Genetic Polymerase Chain Reaction RNA Polymerase I/metabolism RNA Precursors/metabolism RNA Processing, Post-Transcriptional RNA, Ribosomal, 18S/metabolism Ribonucleoproteins/metabolism Ribosomes/metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription, Genetic/physiology
Chemicals
Chromatin DNA Primers RNA Precursors RNA, Ribosomal, 18S Ribonucleoproteins Saccharomyces cerevisiae Proteins RNA Polymerase I
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gallagher Jennifer E G
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520-8024, USA.
Dunbar David A
Granneman Sander
Mitchell Brianna M
Osheim Yvonne
Beyer Ann L
Baserga Susan J
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1226604
Published
2004-10-15
Pages
2506-17
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC529538
Subset
IM
Grants
NIGMS NIH HHS · R01 GM052581 · United States
NIGMS NIH HHS · GM 52581 · United States
External Links
PubMed source DOI: 10.1101/gad.1226604 PMC Full Text View journal details
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