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PMID: 2663473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.

The EMBO journal ·Vol. 8 ·No. 4 ·1989-04-00 ·Pages 1265-70

Sartorius J, Lehming N, Kisters B, von Wilcken-Bergmann B, Müller-Hill B

Abstract

Several lac repressor mutants have been isolated which repress beta-galactosidase synthesis in Escherichia coli up to 200-fold. They do so by binding specifically to particular symmetrical lac Oc operator variants. The mutations in the lac repressor are localized in two separate parts of the recognition helix comprising (i) residues 1 and 2 which interact with base pairs 4 and 5 of lac operator and (ii) residue 6 which recognizes operator base pair 6. Mutations of residues 1 and 2 may be combined with a mutation of residue 6. The resulting mutant protein binds specifically to an operator variant with three symmetric exchanges in base pairs 4, 5 and 6.

MeSH Terms
Base Sequence Binding Sites DNA, Bacterial/genetics Escherichia coli/genetics,metabolism Genetic Variation Lac Operon Mutation Protein Conformation Repressor Proteins/genetics,metabolism Transcription Factors/genetics
Chemicals
DNA, Bacterial Repressor Proteins Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sartorius J
Institut für Genetik der Universität zu Köln, FRG.
Lehming N
Kisters B
von Wilcken-Bergmann B
Müller-Hill B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-04-00
Pages
1265-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400943
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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