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PMID: 26139822 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Salt-induced transcription factor MYB74 is regulated by the RNA-directed DNA methylation pathway in Arabidopsis.

Journal of experimental botany ·Vol. 66 ·No. 19 ·2015-09-00 ·Pages 5997-6008

Xu R, Wang Y, Zheng H, Lu W, Wu C, Huang J, Yan K, Yang G, Zheng C

Abstract

Salt stress is one of the major abiotic stresses in agriculture worldwide that causes crop failure by interfering with the profile of gene expression and cell metabolism. Transcription factors and RNA-directed DNA methylation (RdDM) play an important role in the regulation of gene activation under abiotic stress in plants. This work characterized AtMYB74, a member of the R2R3-MYB gene family, which is transcriptionally regulated mainly by RdDM as a response in salt stress in Arabidopsis. Bisulphite sequencing indicated that 24-nt siRNAs target a region approximately 500bp upstream of the transcription initiation site of AtMYB74, which is heavily methylated. Levels of DNA methylation in this region were significantly reduced in wild type plants under salt stress, whereas no changes were found in RdDM mutants. Northern blot and quantitative real-time reverse transcription PCR analysis showed that the accumulation of 24-nt siRNAs was decreased in WT plants under salt stress. Further promoter deletion analysis revealed that the siRNA target region is essential for maintaining AtMYB74 expression patterns. In addition, transgenic plants overexpressing AtMYB74 displayed hypersensitivity to NaCl during seed germination. These results suggest that changes in the levels of the five 24-nt siRNAs regulate the AtMYB74 transcription factor via RdDM in response to salt stress.

Keywords
Arabidopsis AtMYB74 RNA-directed DNA methylation salt stress siRNA transcription transcription factor.
MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,physiology Arabidopsis Proteins/genetics,metabolism DNA Methylation Gene Expression Regulation, Plant/drug effects Plants, Genetically Modified/genetics,metabolism RNA, Plant/genetics,metabolism RNA, Small Interfering/genetics,metabolism Salt Tolerance Sequence Alignment Sodium Chloride/pharmacology Transcription Factors/genetics,metabolism
Chemicals
Arabidopsis Proteins MYB74 protein, Arabidopsis RNA, Plant RNA, Small Interfering Transcription Factors Sodium Chloride
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Xu Rui
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.
Wang Yuhan
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Zheng Hao
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Lu Wei
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Wu Changai
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Huang Jinguang
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Yan Kang
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Yang Guodong
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Zheng Chengchao
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China cczheng@sdau.edu.cn.
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Article Info
Journal
Journal of experimental botany
Abbr.
J Exp Bot
ISSN
1460-2431
DOI
10.1093/jxb/erv312
Email
cczheng@sdau.edu.cn
Published
2015-09-00
Epub
2015-00-02
Pages
5997-6008
Language
English
Region
England
NLM ID
9882906
PMCID
PMC4566987
Subset
IM
External Links
PubMed source DOI: 10.1093/jxb/erv312 PMC Full Text View journal details
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