Abstract
Plant small RNAs are emerging as significant components of epigenetic processes and of gene networks involved in development and homeostasis. In this paper, to identify small RNAs in wheat, 2,076 small RNAs were identified in a small RNA library from leaf, root, and spike. These small RNAs mapped to non-coding regions the CDS region of protein-coding genes and 5' UTR and 3' UTR regions. The expression of small RNAs in seedling leaves, roots, and spikes were analyzed by northern blot, which indicates that some small RNAs were responsive to abiotic stress treatments including heat, cold, salt and dehydration.
MeSH Terms
Blotting, Northern
Gene Expression Regulation, Plant
Nucleotides/genetics
Plant Leaves/genetics
RNA, Small Interfering/genetics,metabolism
RNA, Untranslated/genetics,metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stress, Physiological/genetics
Triticum/genetics
Chemicals
Nucleotides
RNA, Small Interfering
RNA, Untranslated
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Yao Yingyin
Key Laboratory of Crop Heterosis and Utilization (MOE) and State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100094, China.
Ni Zhongfu
Peng Huiru
Sun Fenglong
Xin Mingming
Sunkar Ramanjulu
Zhu Jian-Kang
Sun Qixin
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