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PMID: 20217168 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Non-coding small RNAs responsive to abiotic stress in wheat (Triticum aestivum L.).

Functional & integrative genomics ·Vol. 10 ·No. 2 ·2010-05-00 ·Pages 187-90

Yao Y, Ni Z, Peng H, Sun F, Xin M, Sunkar R, Zhu JK, Sun Q

Abstract

Plant small RNAs are emerging as significant components of epigenetic processes and of gene networks involved in development and homeostasis. In this paper, to identify small RNAs in wheat, 2,076 small RNAs were identified in a small RNA library from leaf, root, and spike. These small RNAs mapped to non-coding regions the CDS region of protein-coding genes and 5' UTR and 3' UTR regions. The expression of small RNAs in seedling leaves, roots, and spikes were analyzed by northern blot, which indicates that some small RNAs were responsive to abiotic stress treatments including heat, cold, salt and dehydration.

MeSH Terms
Blotting, Northern Gene Expression Regulation, Plant Nucleotides/genetics Plant Leaves/genetics RNA, Small Interfering/genetics,metabolism RNA, Untranslated/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Stress, Physiological/genetics Triticum/genetics
Chemicals
Nucleotides RNA, Small Interfering RNA, Untranslated
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Yao Yingyin
Key Laboratory of Crop Heterosis and Utilization (MOE) and State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100094, China.
Ni Zhongfu
Peng Huiru
Sun Fenglong
Xin Mingming
Sunkar Ramanjulu
Zhu Jian-Kang
Sun Qixin
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Article Info
Journal
Functional & integrative genomics
Abbr.
Funct Integr Genomics
ISSN
1438-7948
Published
2010-05-00
Epub
2010-00-09
Pages
187-90
Language
English
Region
Germany
NLM ID
100939343
PMCID
PMC3059194
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059138 · United States
NIGMS NIH HHS · R01 GM059138-15 · United States
NIGMS NIH HHS · R01 GM070795 · United States
NIGMS NIH HHS · R01 GM070795-09 · United States
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