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PMID: 26119570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Discovery of microbial natural products by activation of silent biosynthetic gene clusters.

Nature reviews. Microbiology ·Vol. 13 ·No. 8 ·2015-08-00 ·Pages 509-23

Rutledge PJ, Challis GL

Abstract

Microorganisms produce a wealth of structurally diverse specialized metabolites with a remarkable range of biological activities and a wide variety of applications in medicine and agriculture, such as the treatment of infectious diseases and cancer, and the prevention of crop damage. Genomics has revealed that many microorganisms have far greater potential to produce specialized metabolites than was thought from classic bioactivity screens; however, realizing this potential has been hampered by the fact that many specialized metabolite biosynthetic gene clusters (BGCs) are not expressed in laboratory cultures. In this Review, we discuss the strategies that have been developed in bacteria and fungi to identify and induce the expression of such silent BGCs, and we briefly summarize methods for the isolation and structural characterization of their metabolic products.

MeSH Terms
Bacteria/genetics,metabolism Bacterial Proteins/chemistry,genetics,metabolism Biological Products/chemistry,metabolism Gene Expression Regulation, Bacterial/physiology Multigene Family Silencer Elements, Transcriptional/genetics
Chemicals
Bacterial Proteins Biological Products
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rutledge Peter J
School of Chemistry, The University of Sydney, New South Wales 2006, Australia.
Challis Gregory L
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
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Article Info
Journal
Nature reviews. Microbiology
Abbr.
Nat Rev Microbiol
ISSN
1740-1534
DOI
10.1038/nrmicro3496
Published
2015-08-00
Epub
2015-00-29
Pages
509-23
Language
English
Region
England
NLM ID
101190261
Subset
IM
Grants
Medical Research Council · MR/N501839/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/L010852/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/M017982/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/K002341/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/L023342/1) · United Kingdom
External Links
PubMed source DOI: 10.1038/nrmicro3496 View journal details
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