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PMID: 21327279 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler.

Molecular bioSystems ·Vol. 7 ·No. 4 ·2011-04-00 ·Pages 1056-9

Shao Z, Luo Y, Zhao H

Abstract

We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.

MeSH Terms
Bacteria/genetics,metabolism Biological Products/genetics Biosynthetic Pathways/genetics Genetic Engineering/methods Genetic Vectors/genetics,metabolism Recombination, Genetic Saccharomyces cerevisiae/genetics,metabolism Synthetic Biology
Chemicals
Biological Products
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shao Zengyi
Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Luo Yunzi
Zhao Huimin
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Article Info
Journal
Molecular bioSystems
Abbr.
Mol Biosyst
ISSN
1742-2051
DOI
10.1039/c0mb00338g
Published
2011-04-00
Epub
2011-00-16
Pages
1056-9
Language
English
Region
England
NLM ID
101251620
PMCID
PMC4320646
Subset
IM
Grants
NIGMS NIH HHS · P01 GM077596 · United States
NIGMS NIH HHS · GM077596 · United States
External Links
PubMed source DOI: 10.1039/c0mb00338g PMC Full Text
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