Abstract
Fungi produce numerous low molecular weight molecules endowed with a multitude of biological activities. However, mining the full-genome sequences of fungi indicates that their potential to produce secondary metabolites is greatly underestimated. Because most of the biosynthesis gene clusters are silent under laboratory conditions, one of the major challenges is to understand the physiological conditions under which these genes are activated. Thus, we cocultivated the important model fungus Aspergillus nidulans with a collection of 58 soil-dwelling actinomycetes. By microarray analyses of both Aspergillus secondary metabolism and full-genome arrays and Northern blot and quantitative RT-PCR analyses, we demonstrate at the molecular level that a distinct fungal-bacterial interaction leads to the specific activation of fungal secondary metabolism genes. Most surprisingly, dialysis experiments and electron microscopy indicated that an intimate physical interaction of the bacterial and fungal mycelia is required to elicit the specific response. Gene knockout experiments provided evidence that one induced gene cluster codes for the long-sought after polyketide synthase (PKS) required for the biosynthesis of the archetypal polyketide orsellinic acid, the typical lichen metabolite lecanoric acid, and the cathepsin K inhibitors F-9775A and F-9775B. A phylogenetic analysis demonstrates that orthologs of this PKS are widespread in nature in all major fungal groups, including mycobionts of lichens. These results provide evidence of specific interaction among microorganisms belonging to different domains and support the hypothesis that not only diffusible signals but intimate physical interactions contribute to the communication among microorganisms and induction of otherwise silent biosynthesis genes.
MeSH Terms
Actinobacteria/classification,physiology,ultrastructure
Aspergillus nidulans/genetics,metabolism,ultrastructure
Blotting, Northern
Chromatography, High Pressure Liquid
Ecosystem
Fungal Proteins/genetics,metabolism
Gene Expression Profiling
Gene Expression Regulation, Fungal
Genome, Fungal/genetics
Macrolides/metabolism
Microscopy, Electron, Scanning
Molecular Structure
Mutation
Mycelium/genetics,metabolism,ultrastructure
Oligonucleotide Array Sequence Analysis
Phylogeny
Polyketide Synthases/classification,genetics,metabolism
Reverse Transcriptase Polymerase Chain Reaction
Salicylates/analysis,chemistry
Zearalenone/chemistry,metabolism
Chemicals
Fungal Proteins
Macrolides
Salicylates
lecanoric acid
Zearalenone
Polyketide Synthases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Schroeckh Volker
Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, 07745 Jena, Germany.
Scherlach Kirstin
Nützmann Hans-Wilhelm
Shelest Ekaterina
Schmidt-Heck Wolfgang
Schuemann Julia
Martin Karin
Hertweck Christian
Brakhage Axel A
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