Home LiteratureArticle Details
PMID: 2582363 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rat alpha 1-fetoprotein messenger RNA: 5'-end sequence and glucocorticoid-suppressed liver transcription in an improved nuclear run-off assay.

Nucleic acids research ·Vol. 13 ·No. 7 ·1985-04-11 ·Pages 2387-98

Turcotte B, Guertin M, Chevrette M, Bélanger L

Abstract

Cloned cDNA fragments spanning nearly the entire coding regions of rat AFP and albumin genes were used in liver nuclear run-off assays. Under standard assay conditions, transcription signals detected with 5' probes were systematically stronger than with 3' probes. Heparin eliminated this phenomenon, which suggests that nuclear run-off assays are subject to in vitro reinitiation occurring preferentially in promoter gene regions. Transcription in the presence of heparin indicates that very few polymerases are engaged on the AFP gene in adult rat liver. Dexamethasone treatment of developing rat liver results in the loss of transcribing polymerases from all regions of the AFP gene. Albumin gene transcription is unaffected. Inhibition of liver protein synthesis with cycloheximide does not modify the AFP gene suppressive action of dexamethasone. Glucocorticoid hormone receptors may thus directly interact with the AFP locus, blocking polymerase initiation. We also report the sequence analysis of rat AFP mRNA, which reveals the existence of two potential initiation codons on this molecule.

MeSH Terms
Albumins/genetics Amino Acid Sequence Animals Base Sequence DNA/analysis DNA-Directed RNA Polymerases/metabolism Dexamethasone/pharmacology Liver/drug effects,metabolism RNA, Messenger/analysis Rats Transcription, Genetic/drug effects alpha-Fetoproteins/genetics
Chemicals
Albumins RNA, Messenger alpha-Fetoproteins Dexamethasone DNA DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Turcotte B
Guertin M
Chevrette M
Bélanger L
References (26)
26 references, click to expand
  1. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  2. Transcriptional regulation of the tryptophan oxygenase gene in rat liver by glucocorticoids.
    J Biol Chem. 1983 Apr 25;258(8):4750-3 PMID: 6187742
  3. Rapid suppression of alpha 1-fetoprotein gene transcription by dexamethasone in developing rat liver.
    Biochemistry. 1983 Aug 30;22(18):4296-302 PMID: 6194818
  4. Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed region.
    Cell. 1983 Dec;35(2 Pt 1):381-92 PMID: 6317184
  5. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  6. Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo.
    Nature. 1984 Mar 15-21;308(5956):241-6 PMID: 6700727
  7. Segmental homologies in the coding and 3' non-coding sequences of rat liver cytochrome P-450e and P-450b cDNAs and cytochrome P-450e-like genes.
    Biochem Biophys Res Commun. 1984 Jan 30;118(2):655-62 PMID: 6322758
  8. Differential DNase I sensitivity of the albumin and alpha-fetoprotein genes in chromatin from rat tissues and cell lines.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5031-5 PMID: 6206492
  9. Oncodevelopmental and hormonal regulation of alpha 1-fetoprotein gene expression.
    Adv Enzyme Regul. 1983;21:73-99 PMID: 6085825
  10. Hormonal regulation of alpha1 foetoprotein.
    Nature. 1975 Aug 21;256(5519):657-9 PMID: 50565
  11. Sequence of chicken ovalbumin mRNA.
    Nature. 1978 Jun 29;273(5665):723-8 PMID: 661981
  12. Transcription of structural and intervening sequences in the ovalbumin gene and identification of potential ovalbumin mRNA precursors.
    Cell. 1978 Oct;15(2):671-85 PMID: 719758
  13. Isolation of rat alpha1-fetoprotein messenger RNA from Morris hepatoma 7777.
    Cancer Res. 1979 Jun;39(6 Pt 1):2141-8 PMID: 87261
  14. In vitro transcription by wheat germ ribonucleic acid polymerase II: effects of heparin and role of template integrity.
    Biochemistry. 1979 Oct 16;18(21):4581-8 PMID: 387073
  15. R-looping and structural gene indentification of recombinant DNA.
    Methods Enzymol. 1979;68:454-69 PMID: 396442
  16. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  17. The evolution of alpha-fetoprotein and albumin. II. The structures of the alpha-fetoprotein and albumin genes in the mouse.
    J Biol Chem. 1981 Feb 25;256(4):1960-7 PMID: 6161930
  18. Homology between the primary structure of alpha-fetoprotein, deduced from a complete cDNA sequence, and serum albumin.
    Nature. 1981 May 21;291(5812):201-5 PMID: 6164927
  19. Nucleotide sequence of cloned rat serum albumin messenger RNA.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):243-6 PMID: 7017712
  20. Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3521-5 PMID: 6167988
  21. Clustering of RNA polymerase B molecules in the 5' moiety of the adult beta-globin gene of hen erythrocytes.
    Nucleic Acids Res. 1981 Jun 11;9(11):2589-98 PMID: 6269056
  22. Glucocorticosteroid suppression of alpha1-fetoprotein synthesis in developing rat liver. Evidence for selective gene repression at the transcriptional level.
    Biochemistry. 1981 Nov 10;20(23):6665-72 PMID: 6171301
  23. Hormonal modulation of alpha-fetoprotein gene expression in newborn rat livers.
    Nucleic Acids Res. 1981 Dec 21;9(24):6917-33 PMID: 6174948
  24. Fine structure and evolution of the rat serum albumin gene.
    Mol Cell Biol. 1981 Oct;1(10):871-83 PMID: 6287210
  25. RNA synthesis and number of transcribing polymerase molecules in ischemic liver nuclei.
    Mol Cell Biochem. 1982 Oct 1;48(1):59-63 PMID: 6184606
  26. Primary structures of human alpha-fetoprotein and its mRNA.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4604-8 PMID: 6192439
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.7.2387
Published
1985-04-11
Pages
2387-98
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341162
Subset
IM
Databases
GENBANK
V01254, X02361
External Links
PubMed source DOI: 10.1093/nar/13.7.2387 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2