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PMID: 6174948 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Hormonal modulation of alpha-fetoprotein gene expression in newborn rat livers.

Nucleic acids research ·Vol. 9 ·No. 24 ·1981-12-21 ·Pages 6917-33

Chiu JF, Massari RJ, Schwartz CE, Meisler NT, Thanassi JW

Abstract

Suppression of serum alpha-fetoprotein (AFP) levels in glucocorticoid treated newborn rats was investigated. Daily intraperitoneal injection of 2 micrograms/g body weight of dexamethasone into newborn rats greatly reduced the concentration of AFP in the serum and liver cytosol. In contrast, this treatment stimulated liver ornithine decarboxylase activity. The reduction in AFP levels is not due to a change of distribution of AFP molecular variants, inhibition of secretion of synthesized AFP by the liver or disruption of liver polysomes. Glucocorticoids decrease the AFP levels in hormone-treated rats by supressing the synthesis of AFP. The size of AFP polysomes isolated from the livers of dexamethasone-treated rats were as large as those from normal rats. However, the amount of AFP-producing polysomes in hormone-treated rat liver is only 14% of the controls. By hybridization assays, it was found that dexamethasone treated livers contained decreased amounts of AFP mRNA sequences in liver cytoplasmic and nuclear RNAs. The decreased amounts of AFP mRNA sequences in hormone-treated liver are caused by both a decrease in the rate of AFP mRNA transcription and in AFP mRNA stability.

MeSH Terms
Animals Animals, Newborn Dexamethasone/pharmacology Gene Expression Regulation/drug effects Liver/drug effects,metabolism Polyribosomes/drug effects RNA, Messenger/metabolism Rats alpha-Fetoproteins/biosynthesis,genetics
Chemicals
RNA, Messenger alpha-Fetoproteins Dexamethasone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chiu J F
Massari R J
Schwartz C E
Meisler N T
Thanassi J W
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42 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.24.6917
Published
1981-12-21
Pages
6917-33
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327651
Subset
IM
Grants
NIADDK NIH HHS · AM 25490 · United States
NCI NIH HHS · CA 25098 · United States
External Links
PubMed source DOI: 10.1093/nar/9.24.6917 PMC Full Text View journal details
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