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PMID: 91159 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

alpha 1-Fetoprotein mRNA of rat yolk sac and hepatoma.

Nucleic acids research ·Vol. 7 ·No. 1 ·1979-09-11 ·Pages 239-49

Chiu JF, Decha-Umphai W, Commer P

Abstract

Rat alpha 1-fetoprotein mRNA was isolated and purified to apparent homogeneity by means of immunoadsorption and oligo (dT) cellulose affinity chromatography. Purified AFP mRNA migrated as a 21S peak in 2.5% SDS-polyacrylamide gels. The translation product of this mRNA in micrococcal nuclease treated reticulocyte lysate was identified as AFP by specific immunoprecipitation, SDS-gel electrophoresis and tryptic digestion analysis. DNA complimentary to AFP mRNA was synthesized with avian meyloblastosis virus RNA-dependent DNA polymerase. This AFP cDNA was used as a probe to quantitate AFP mRNA in the developing rat liver and to compare the complexity and diversity of AFP mRNA derived from the normal rat liver and Morris hepatoma 7777. We found that the amount of functional AFP mRNA is decreasing during liver development. There is very little, if any, AFP mRNA in the adult rat liver. A high degree of homology between the AFP mRNA sequences of yolk sac and hepatoma was also found.

MeSH Terms
Animals Base Sequence Female Liver/metabolism Liver Neoplasms, Experimental/metabolism Micrococcal Nuclease Pregnancy Protein Biosynthesis RNA, Messenger/isolation & purification,metabolism Rats Yolk Sac/metabolism alpha-Fetoproteins/biosynthesis
Chemicals
RNA, Messenger alpha-Fetoproteins Micrococcal Nuclease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chiu J F
Decha-Umphai W
Commer P
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.1.239
Published
1979-09-11
Pages
239-49
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328009
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.1.239 PMC Full Text View journal details
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