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PMID: 896479 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

RNA aggregation during sulfhydryl-agarose chromatography of mercurated RNA.

Nucleic acids research ·Vol. 4 ·No. 6 ·1977-06-00 ·Pages 1979-88

Konkel DA, Ingram VM

Abstract

Isolation of newly synthesized mercurated RNA transcripts by chromatography on sulfhydryl-agarose has recently been used to reduce contamination by endogenous RNA derived from the chromatin template. We show that substantial RNA aggregation occurs during standard isolation procedures, causing significant retention of endogenous (unmercurated) RNA on sulfhydryl-agarose. We describe methods to reduce substantially this problem and discuss the implications of our findings for interpretation of previous hybridization and transcription experiments.

MeSH Terms
Animals Chick Embryo Chromatin/metabolism Chromatography, Affinity/methods DNA/metabolism Erythrocytes/metabolism Globins/biosynthesis Mercury Nucleic Acid Hybridization RNA/biosynthesis,isolation & purification RNA, Messenger/metabolism Sepharose Sulfhydryl Compounds Transcription, Genetic Uridine/metabolism
Chemicals
Chromatin RNA, Messenger Sulfhydryl Compounds RNA Globins DNA Sepharose Mercury Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Konkel D A
Ingram V M
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.6.1979
Published
1977-06-00
Pages
1979-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342535
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.6.1979 PMC Full Text View journal details
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