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PMID: 4140516 Published · ppublish English Journal Article

Nonhistone proteins control gene expression in reconstituted chromatin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 5057-61

Barrett T, Maryanka D, Hamlyn PH, Gould HJ

Abstract

Chromatin was reconstituted from the purified DNA and histones of chicken erythrocytes and the nonhistone proteins of either chicken reticulocytes or chicken liver. Reconstituted chromatins, native chicken reticulocyte chromatin, and free DNA were transcribed with Escherichia coli RNA polymerase and the concentrations of globin-specific sequences in the RNA products were measured by hybridization with [(3)H]DNA complementary to chicken globin messenger RNA. Reticulocyte, but not liver, nonhistone proteins were shown to activate the globin genes in reconstituted erythrocyte chromatin. The transcripts of native and reconstituted chromatins were indistinguishable in respect of both the total yield of the RNA and the fractional yield of globin-specific sequences.

MeSH Terms
Animals Cell-Free System Centrifugation, Density Gradient Chickens Chromatin/metabolism DNA/biosynthesis,metabolism Electrophoresis, Polyacrylamide Gel Erythrocytes/metabolism Globins/biosynthesis Histones/metabolism Kinetics Molecular Weight Nucleic Acid Hybridization Nucleoproteins/metabolism RNA, Messenger/biosynthesis RNA-Directed DNA Polymerase/metabolism Reticulocytes/metabolism Templates, Genetic Transcription, Genetic
Chemicals
Chromatin Histones Nucleoproteins RNA, Messenger Globins DNA RNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Barrett T
Maryanka D
Hamlyn P H
Gould H J
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.5057
Published
1974-12-00
Pages
5057-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434039
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.5057 PMC Full Text View journal details
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